Proteomic Study of Renal Uric Acid Stone
Proteomic Study of Renal Uric Acid Stone
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DOI：
10.1016/j.urology.2012.02.019
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发表时间：
2012-08-01
期刊：
影响因子：
2.1
通讯作者：
Tsai, Yuh-Shyan
中科院分区：
文献类型：
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作者：
Jou, Yeong-Chin;Fang, Chiung-Yao;Tsai, Yuh-Shyan
目的采用质谱法分析尿尿酸结石基质蛋白（SMP），探讨尿酸结石形成的机制。SMP在尿路结石形成中起重要作用。一些蛋白质组学研究适用于含钙结石的报道；然而，尿尿酸结石的蛋白质组学研究尚未见报道。方法对5例肾性尿酸结石进行脱矿，分离smp。获得的蛋白质用反相液相色谱-串联质谱进行分析，获得的数据使用Matrix Science， Mascot在Swiss Prot人蛋白数据库中进行检索。鉴定出的蛋白被提交到AmiGO网站进行基因本体分析。将它们提交给Metacore软件和京都基因与基因组百科网站（KEGG）进行通路分析。免疫印迹法验证ms测定的蛋白表达。结果从5个蛋白质组学结果中鉴定出242个蛋白，每个结果鉴定出的蛋白数量在52 ~ 156个之间。Metacore软件分析提示炎症可能在肾尿酸结石形成中起重要作用。内源性代谢途径也被分析并提交给KEGG网站，表明这些蛋白质可能参与脂肪代谢。选择鉴定出的5个蛋白进行免疫印迹验证，确认3个蛋白。结论炎症过程可能在肾尿酸结石形成过程中起一定作用。我们的内源性代谢途径分析数据显示，这些蛋白可能参与脂质代谢。这一发现是否意味着脂毒性与肾尿酸结石之间的关系有待进一步研究。中华泌尿外科杂志（英文版），2012。(c) 2012 Elsevier Inc.
OBJECTIVE To analyze urinary uric acid stone matrix proteins (SMP) with mass spectrometry (MS) to evaluate the mechanisms of uric acid stone formation. SMP plays an important role in urinary stone formation. Several proteomic studies apply to calcium-containing stones have been reported; however no proteomic study for urinary uric acid stone has been reported.METHODS Pure kidney uric acid stones from 5 individuals were demineralized, and SMPs were isolated. The obtained proteins were analyzed with reverse-phase liquid chromatography-tandem MS. The acquired data were searched against a Swiss Prot human protein database using Matrix Science, Mascot. The identified proteins were submitted to the AmiGO Web site for gene ontology analysis. They were also sumitted to Metacore software and Kyoto Encyclopedia of Genes and Genomes website (KEGG) for pathway analysis. MS-determined protein expressions were verified by immunoblot.RESULTS MS analysis identified 242 proteins from 5 proteomic results and the number of the identified protein of each result ranged from 52 to 156. Metacore software analysis suggested that inflammation may play an important role for kidney uric acid stone formation. Endogenous metabolic pathways were also analyzed and submitted to KEGG Web site, which revealed that these proteins may participate in fat metabolism. Five identified proteins were selected for immunoblot validation, and 3 proteins were confirmed.CONCLUSION Our results suggest that inflammatory process may play a role in kidney uric acid stone formation. Our endogenous metabolic pathway analysis data revealed that these proteins may participate in lipid metabolism. Whether this finding implies a relation between lipotoxicity and kidney uric acid stone former requires further investigation. UROLOGY 80: 260-266, 2012. (c) 2012 Elsevier Inc.