The role of a "germinal plasm" in the formation of primordial germ cells in Rana pipiens.
The role of a "germinal plasm" in the formation of primordial germ cells in Rana pipiens.
复制标题
“生殖质”在林蛙原始生殖细胞形成中的作用。
DOI：
10.1016/0012-1606(66)90019-4
复制
发表时间：
1966
影响因子：
2.7
通讯作者：
L.Dennis Smith
中科院分区：
文献类型：
--
作者：
L.Dennis Smith
用紫外线照射尖音蛙卵的植物半球，开始第一次卵裂分裂，剂量大于8000尔格/mm 2，导致100%的幼虫完全消除原始生殖细胞，否则明显正常。用同等剂量的紫外线照射动物半球对原始生殖细胞的形成没有明显的影响。紫外线对生殖细胞形成的影响在第一次分裂时最大。在此之后，越来越少的卵，在植物半球被照射，发育成没有生殖细胞的幼虫。在8细胞期，未观察到生殖细胞的完全消除。通过将未辐照鸡蛋的植物半球细胞质注射到辐照鸡蛋的植物半球中，获得辐照鸡蛋中生殖系的恢复，但通过注射动物半球细胞质，未发生恢复。生殖细胞灭活的紫外作用光谱表明敏感物质是核酸。这些结果与一个特定的细胞质germinate决定簇的假设是一致的。这个决定子只存在于植物半球的细胞质中，它可能是一种核酸，并且在第一次卵裂分裂之前出现在胚胎发育中。
Ultraviolet irradiation of the vegetal hemisphere of Rana pipiens eggs, beginning the first cleavage division, with doses greater than 8000 ergs/mm2resulted in the complete elimination of primordial germ cells in 100% of the larvae that were otherwise apparently normal. Irradiation of the animal hemisphere with equivalent doses of UV had no apparent effect on the formation of primordial germ cells. The effect of UV on germ cell formation was greatest at the time of the first division. After this, increasingly fewer eggs, which had been irradiated at the vegetal hemisphere, developed into larvae without germ cells. At the 8-cell stage, complete elimination of germ cells was not observed. Restitution of the germ line in irradiated eggs was obtained by injecting vegetal hemisphere cytoplasm from unirradiated eggs into the vegetal hemisphere of irradiated eggs, but restitution did not occur by injection of animal hemisphere cytoplasm. The UV action spectrum of germ cell inactivation suggests that the sensitive material is a nucleic acid. These results are consistent with the hypothesis of a specific cytoplasmic germinal determinant. This determinant is present only in the vegetal hemisphere cytoplasm; it is probably a nucleic acid; and it appears in embryonic development prior to the first cleavage division.