A search for novel markers of alcohol abuse using magnetic beads and MALDI-TOF/TOF mass spectrometry
A search for novel markers of alcohol abuse using magnetic beads and MALDI-TOF/TOF mass spectrometry
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DOI：
10.1002/prca.200800174
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发表时间：
2009-07-01
影响因子：
2
通讯作者：
Nomura, Fumio
中科院分区：
文献类型：
--
作者：
Sogawa, Kazuyuki;Satoh, Mamoru;Nomura, Fumio
需要客观指标来评估过度饮酒，这可能导致各种医疗和社会问题。在这项研究中，我们使用ClinProt （TM）系统（由磁珠和MALDI-TOF/TOF MS组成）进行了血清肽分析，以在16名住院接受康复计划的慢性酒精患者中发现新的酒精滥用生物标志物。在禁欲期间，共有22个峰值发生了显著变化。在这22个峰中，有3个峰的m/z小于或等于3000，并且峰强度很大，我们进行了MS/MS分析，然后进行了MASCOT搜索。1466 Da和1616 Da肽表达上调。并分别被鉴定为纤维蛋白肽A和磷酸化纤维蛋白肽A的片段。另一方面，2660 Da肽在入院时下调，在戒断期间增加，被确定为纤维蛋白原α - C链的一个片段。SELDI-TOF MS ProteinChip (R)系统分析未检测到这些峰。在谷氨酰转移酶无应答者中，酒精滥用引起的这些峰的改变也可见。这些蛋白质片段可能是过量饮酒的额外生物标志物。
Objective markers are required to assess excessive alcohol consumption, which can lead to a various medical and social problems. In this study, we carried out serum peptidome analyses using the ClinProt (TM) system, which consists of magnetic beads and MALDI-TOF/TOF MS, to find novel biomarkers of alcohol abuse in 16 chronic alcoholic patients that were hospitalized for a rehabilitation program. A total of 22 peaks were found to be significantly altered during abstinence. Out of these 22 peaks, 3 peaks that had an m/z of 3000 or less and substantial peak intensities were subjected to MS/MS analysis followed by a MASCOT search. The 1466 Da and the 1616 Da peptides were upregulated. on admission and were identified as fragments of fibrinopeptide A and phosphorylated fibrinopeptide A, respectively. On the other hand, the 2660 Da peptide, which was downregulated on admission and increased during abstinence, was identified as a fragment of the fibrinogen alpha C chain. These peaks were not detectable by the SELDI-TOF MS ProteinChip (R) system analysis. The alterations in these peaks induced by alcohol abuse were also seen in gamma glutamyltransferase nonresponders. These protein fragments may be additional biomarkers for excessive alcohol drinking.