Transcriptomic differences between day and night in Acropora millepora provide new insights into metabolite exchange and light-enhanced calcification in corals
Transcriptomic differences between day and night in Acropora millepora provide new insights into metabolite exchange and light-enhanced calcification in corals
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DOI：
10.1111/mec.13328
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发表时间：
2015-09-01
影响因子：
4.9
通讯作者：
Miller, D. J.
中科院分区：
文献类型：
--
作者：
Bertucci, A.;Foret, S.;Miller, D. J.
造礁珊瑚的进化成功通常归因于它们与共生藻属的光合腰鞭毛虫的共生关系，但伴侣之间的代谢相互作用和光增强钙化（LEC）的分子基础尚不清楚。在这里，珊瑚Acropora millepora和甲藻共生体之间的相互作用的代谢基础进行了研究，通过比较基因表达水平在整个转录组水平的光和暗条件下。在鉴定的497个差异表达基因中，发现一组参与胆固醇转运的基因在光照条件下上调，证实了这种化合物在珊瑚共生中的重要性。虽然离子转运蛋白可能在钙化中发挥作用，但在本研究中没有差异表达，但与骨骼有机基质组成和组织相关的许多基因的表达水平在光照条件下较高。这意味着有机基质合成的速率是限制夜间钙化的一个因素。因此，白天的LEC很可能是由于光合作用活动导致基质合成和前体分子供应增加的结果。
The evolutionary success of reef-building corals is often attributed to their symbiotic relationship with photosynthetic dinoflagellates of the genus Symbiodinium, but metabolic interactions between the partners and the molecular bases of light-enhanced calcification (LEC) are not well understood. Here, the metabolic bases of the interaction between the coral Acropora millepora and its dinoflagellate symbiont were investigated by comparing gene expression levels under light and dark conditions at the whole transcriptome level. Among the 497 differentially expressed genes identified, a suite of genes involved in cholesterol transport was found to be upregulated under light conditions, confirming the significance of this compound in the coral symbiosis. Although ion transporters likely to have roles in calcification were not differentially expressed in this study, expression levels of many genes associated with skeletal organic matrix composition and organization were higher in light conditions. This implies that the rate of organic matrix synthesis is one factor limiting calcification at night. Thus, LEC during the day is likely to be a consequence of increases in both matrix synthesis and the supply of precursor molecules as a result of photosynthetic activity.