Molecular Signature of Human amniotic Fluid Stem Cells During Fetal Development
Molecular Signature of Human amniotic Fluid Stem Cells During Fetal Development
复制标题
DOI：
10.2174/1574888x11308010009
复制
发表时间：
2013-01-01
影响因子：
2.7
通讯作者：
Guillot, Pascale V.
中科院分区：
文献类型：
--
作者：
Moschidou, Dafni;Drews, Katharina;Guillot, Pascale V.
妊娠中期c-KIT+羊水干细胞（AFSC）具有介于胚胎干细胞和成体干细胞之间的中间表型，并且易于重编程为多能性。我们之前的研究表明，妊娠早期的AFSC可以通过无转基因的方法重新编程为功能性多能性。尽管两个亲本群体具有共同的表型，表达CD29、CD44、CD73、CD90、CD105、SSEA4和OCT4，但与妊娠早期的细胞相反，妊娠中期的AFSC不表达NANOG、SSEA3、TRA-1-60和TRA-1-81，并且生长动力学较慢。在这里，我们使用Illumina Beadstudio微阵列平台分析了妊娠早期和晚期AFSC的转录组，发现了一个独特的妊娠早期AFSC特异性基因表达特征，包括366个基因和更大的603个与hESC共同的基因，而妊娠中期AFSC和hESC之间有496个基因重叠。我们得出结论，这两个种群是相关的，但彼此之间以及与hESC不同。
Mid-gestation c-KIT+ amniotic fluid stem cells (AFSC) have an intermediate phenotype between embryonic and adult stem cells and are easy to reprogram to pluripotency. We previously showed that 1st trimester AFSC can be reprogrammed to functional pluripotency in a transgene-free approach. Despite both parental populations sharing a common phenotype, expressing CD29, CD44, CD73, CD90, CD105, SSEA4 and OCT4, 2nd trimester AFSC, contrary to 1st trimester cells, do not express NANOG, SSEA3, TRA-1-60 and TRA-1-81, and have slower growth kinetics. Here, we used the Illumina Beadstudio microarray platform to analyse the transcriptome of 1st and 2nd trimester AFSC and show a unique 1st trimester AFSC-specific gene expression signature consisting of 366 genes and a larger set of 603 genes common with hESC compared to 496 genes overlapping between 2nd trimester AFSC and hESC. We conclude that both populations are related but distinct to each other as well as to hESC.