High sensitivity point-of-care device for direct virus diagnostics
High sensitivity point-of-care device for direct virus diagnostics
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DOI：
10.1016/j.bios.2013.05.046
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发表时间：
2013-11-15
影响因子：
12.6
通讯作者：
Rozlosnik, Noemi
中科院分区：
文献类型：
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作者：
Kiilerich-Pedersen, Katrine;Dapra, Johannes;Rozlosnik, Noemi
流感感染与高发病率和高死亡率相关，存在引发大流行的风险，并在全球范围内造成相当大的经济负担。为了改善对该疾病的管理，在现场或患者床边使用准确且快速的即时诊断工具至关重要。传统的诊断方法耗时、昂贵且需要专门的实验室设施。我们提出了一个高灵敏度、高特异性且低成本的平台，能够在不到15分钟内检测急性病毒感染，并以甲型流感病毒（H1N1）为例说明了其可用性。开发了一种带有功能化导电聚合物（PEDOT - OH:TsO）微电极阵列的全聚合物微流体系统，并将其用于对完整的甲型流感病毒（H1N1）颗粒进行无标记实时电化学检测。对甲型流感病毒（H1N1）具有亲和力的DNA适配体被共价连接到微流体通道中的导电聚合物微电极上。基于当病毒粒子被固定探针捕获时阻抗的变化，我们能够检测唾液中具有临床相关浓度的甲型流感病毒（H1N1）。这是一个用于检测和诊断完整病毒颗粒的新型、稳定且非常灵敏的即时诊断平台。（C）2013爱思唯尔有限公司。保留所有权利。
Influenza infections are associated with high morbidity and mortality, carry the risk of pandemics, and pose a considerable economic burden worldwide. To improve the management of the illness, it is essential with accurate and fast point-of-care diagnostic tools for use in the field or at the patient's bedside. Conventional diagnostic methods are time consuming, expensive and require specialized laboratory facilities. We present a highly sensitive, highly specific, and low cost platform to test for acute virus infections in less than 15 mm, employing influenza A virus (H1N1) as an example of its usability. An all polymer microfluidic system with a functionalized conductive polymer (PEDOT-OH:TsO) microelectrode array was developed and exploited for label free and real time electrochemical detection of intact influenza A virus (H1N1) particles. DNA aptamers with affinity for influenza A virus (H1N1) were linked covalently to the conductive polymer microelectrodes in the microfluidic channel. Based on changes in the impedance when virions were captured by immobilized probes, we could detect clinically relevant concentrations of influenza A virus (H1N1) in saliva. This is a new, stable and very sensitive point-of-care platform for detection and diagnostics of intact virus particles. (C) 2013 Elsevier B.V. All rights reserved.