Preferential clustering of viral DNA sequences at or near the site of chromosomal rearrangement in fowl adenovirus type 1 DNA-transformed cell lines
Preferential clustering of viral DNA sequences at or near the site of chromosomal rearrangement in fowl adenovirus type 1 DNA-transformed cell lines
复制标题
禽腺病毒 1 型 DNA 转化细胞系中病毒 DNA 序列在染色体重排位点或附近的优先聚集
作者：
M. Ishibashi;T. Yosida;H. Yasue
通过将鸡腺病毒1型（CELO病毒）DNA或其片段转化到正常核型的大鼠细胞系中获得的所有六个转化体具有超过50拷贝当量的病毒DNA序列。每个转化体具有几乎全部（如果不是全部的话）这些病毒DNA序列簇集在标记染色体上。尽管标记染色体在不同细胞系间存在差异，但病毒DNA序列优先聚集在G带标记染色体的无色（或淡染）区域或附近，在该区域发生染色体重排或易位。这些结果表明，不需要特定的染色体作为病毒DNA的整合位点来转化细胞，但在病毒DNA序列簇处或附近的染色体重排可能有助于转化。
All six transformants obtained by inoculating fowl adenovirus type 1 (CELO virus) DNA or its fragments into a rat cell line of normal karyotype had more than 50 copy-equivalents of viral DNA sequences. Each of the transformants had almost all if not all of these viral DNA sequences clustered on a marker chromosome(s). Although the marker chromosome(s) differed from one cell line to another, viral DNA sequences preferentially clustered in or near the achromatic (or light-stained) region of the G-banded marker chromosomes where chromosomal rearrangement or translocation occurred. These results indicate that no particular chromosome is required to act as the integration site of viral DNA for the transformation of cells, but chromosomal rearrangement at or near the cluster of viral DNA sequences might contribute to the transformation.