Chromatin fiber folding: Requirement for the histone H4N-terminal tail
Chromatin fiber folding: Requirement for the histone H4N-terminal tail
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DOI：
10.1016/s0022-2836(03)00025-1
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发表时间：
2003-03-14
影响因子：
5.6
通讯作者：
Richmond, TJ
中科院分区：
文献类型：
--
作者：
Dorigo, B;Schalch, T;Richmond, TJ
我们开发了一种核小体阵列自组装系统，其中重组的、翻译后未修饰的组蛋白与特定序列的 DNA 结合，形成染色质高阶结构。获得的核小体阵列高度均质，在 1 mM 或 100 mM MgCl2 中最大折叠时在 53 S 下沉积。折叠性能与现有系统相当。分析超速离心用于确定单个组蛋白尾部结构域缺失对阵列折叠的影响。通过删除除 H4 N 末端之外的任何一个组蛋白尾，获得完全压实的染色质纤维。 H4 尾部介导压缩的区域位于氨基酸 14-19 的区域。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
We have developed a self-assembly system for nucleosome arrays in which recombinant, post-translationally unmodified histone proteins are combined with DNA of defined-sequence to form chromatin higher-order structure. The nucleosome arrays obtained are highly homogeneous and sediment at 53 S when maximally folded in 1 mM or 100 mM MgCl2. The folding properties are comparable to established systems. Analytical ultracentrifugation is used to determine the consequence of individual histone tail domain deletions on array folding. Fully compacted chromatin fibers are obtained with any one of the histone tails deleted with the exception of the H4 N terminus. The region of the H4 tail, which mediates compaction, resides in the stretch of amino acids 14-19. (C) 2003 Elsevier Science Ltd. All rights reserved.