The Tig1 Histone Deacetylase Complex Regulates Infectious Growth in the Rice Blast Fungus Magnaporthe oryzae
The Tig1 Histone Deacetylase Complex Regulates Infectious Growth in the Rice Blast Fungus Magnaporthe oryzae
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DOI：
10.1105/tpc.110.074302
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发表时间：
2010-07-01
期刊：
影响因子：
11.6
通讯作者：
Xu, Jin-Rong
中科院分区：
文献类型：
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作者：
Ding, Sheng-Li;Liu, Wende;Xu, Jin-Rong
稻瘟病菌是水稻上最具危害性的病原真菌。在这项研究中，我们的特点是TIG1转导β样基因所需的感染性生长和其相互作用的基因，植物感染所需的植物病原真菌在这个模型。在酵母和哺乳动物细胞中的Tig1同源物是保守的组蛋白去乙酰化酶（HDAC）转录辅阻遏物复合物的一部分。tig1缺失突变体无致病性且分生孢子发生缺陷。它对氧化胁迫的敏感性增加，并且不能在植物细胞中形成侵入性菌丝。使用亲和纯化和免疫共沉淀试验，我们确定了几个Tig1相关蛋白，包括两个HDAC是同源的酵母Set3复合物的组成部分。功能分析显示，TIG1、SET3、SNT1和HOS2是M.米。set3，snt1，和hos2缺失突变体显示类似的缺陷中观察到的tig1突变体，但删除HST 1或HOS4没有检测到的表型。Tig1复合物的任何这些核心组分的缺失导致HDAC活性的显著降低。我们的研究结果表明，TIG1，像它的假定的酵母和哺乳动物的直系同源物，是一个保守的HDAC复合物，是所需的感染性生长和分生孢子在M。强调染色质修饰是植物感染过程中一种重要的调控机制。
Magnaporthe oryzae is the most damaging fungal pathogen of rice (Oryza sativa). In this study, we characterized the TIG1 transducin beta-like gene required for infectious growth and its interacting genes that are required for plant infection in this model phytopathogenic fungus. Tig1 homologs in yeast and mammalian cells are part of a conserved histone deacetylase (HDAC) transcriptional corepressor complex. The tig1 deletion mutant was nonpathogenic and defective in conidiogenesis. It had an increased sensitivity to oxidative stress and failed to develop invasive hyphae in plant cells. Using affinity purification and coimmunoprecipitation assays, we identified several Tig1-associated proteins, including two HDACs that are homologous to components of the yeast Set3 complex. Functional analyses revealed that TIG1, SET3, SNT1, and HOS2 were core components of the Tig1 complex in M. oryzae. The set3, snt1, and hos2 deletion mutants displayed similar defects as those observed in the tig1 mutant, but deletion of HST1 or HOS4 had no detectable phenotypes. Deletion of any of these core components of the Tig1 complex resulted in a significant reduction in HDAC activities. Our results showed that TIG1, like its putative yeast and mammalian orthologs, is one component of a conserved HDAC complex that is required for infectious growth and conidiogenesis in M. oryzae and highlighted that chromatin modification is an essential regulatory mechanism during plant infection.