Tomato Zonate Spot Virus Induced Hypersensitive Resistance via an Auxin-Related Pathway in Pepper.
Tomato Zonate Spot Virus Induced Hypersensitive Resistance via an Auxin-Related Pathway in Pepper.
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DOI：
10.1016/j.gene.2022.146320
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发表时间：
2022-02
期刊：
影响因子：
3.5
通讯作者：
Li-Hua Zhao;Lizhen Zhang;Zhong-Ze Hu;Bowen Li;Xue-gang Zheng;Runshuang Qiu;Yue‐Ying Chen;Jing Li;J. Dong;Zhong-kai Zhang
中科院分区：
文献类型：
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作者：
Li-Hua Zhao;Lizhen Zhang;Zhong-Ze Hu;Bowen Li;Xue-gang Zheng;Runshuang Qiu;Yue‐Ying Chen;Jing Li;J. Dong;Zhong-kai Zhang
番茄带状斑病毒（Tomato Zonate Spotvirus，TPEV）在云南省及周边地区的许多粮食和观赏作物上造成重大损失。辣椒（Capsicum chinensePI 152225）在感染TGV后可通过一种迄今未知的机制产生过敏性抗性。转录组数据集显示，从6个文库中共获得了45.81 GB的干净数据，并且映射到辣椒基因组的平均读数百分比超过90.00%。共获得1403个差异表达基因（DEG），其中显著上调基因825个，下调基因578个。基因本体（GO）和京都基因和基因组百科全书（KEGG）途径分析表明，大多数上调的DEG参与基础防御。利用RT-qPCR和病毒诱导的基因沉默（virusinducedgene silencing，VIGS）技术，从71个差异表达基因（DEGs）中初步鉴定出BBC_22506和BBC_18917在辣椒（PI 152225）抗病毒感染的过敏反应（hypersensitivesresponse，HR）中起关键作用。这是首次对辣椒对病毒病防御反应中生长素抗性机制的研究，为进一步研究辣椒对TTV的致病机制以及辣椒对TTV的抗性机制奠定了基础。
Tomato zonate spotvirus (TZSV) often incurs significant losses in many food and ornamental crops in Yunnan province, China, and the surrounding areas. The pepper (Capsicum chinensePI152225)can develop hypersensitive resistance following infection with TZSV, through an as yet unknown mechanism. The transcriptome dataset showed a total of 45.81 GB of clean data were obtained from six libraries, and the average percentage of the reads mapped to the pepper genome was over 90.00 %. A total of 1403 differentially expressed genes (DEGs) were obtained after TZSV infection, including 825significantly up-regulated genes and 578 down-regulated genes. The Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses indicated that most up-regulated DEGs were involved in basal defenses. RT-qPCR, and virus induced gene silencing (VIGS) were used preliminarily to identifyBBC_22506 and BBC_18917, among total of 71 differentially expressed genes (DEGs), that play a key role in mediating the auxin-induced signaling pathway that might take part in hypersensitive response (HR) conferred resistance to viral infection in pepper (PI152225) byTZSV. This is the first study on the mechanism of auxin resistance, involved in defense responses of pepper against viral diseases, which lay the foundation for further study on the pathogenic mechanism of TZSV, as well as the mechanism of resistance to TZSV, in peppers.