Effect of Dachaihu decoction on non-alcoholic fatty liver disease model rats induced by a high-fat high-sugar diet
Effect of Dachaihu decoction on non-alcoholic fatty liver disease model rats induced by a high-fat high-sugar diet
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DOI：
10.1016/j.jtcms.2018.10.001
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发表时间：
2018-10
影响因子：
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通讯作者：
Lu Zhou;Jia-Min Yang;Min Wang;Yu-Shan Gao;Shu-jing Zhang;Yan Sun;Qian Wang;Jian Cui;Yuhang Li
中科院分区：
文献类型：
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作者：
Lu Zhou;Jia-Min Yang;Min Wang;Yu-Shan Gao;Shu-jing Zhang;Yan Sun;Qian Wang;Jian Cui;Yuhang Li
目的建立符合非酒精性脂肪性肝病（NAFLD）发生、发展规律的动物模型，观察大柴胡汤（DD）对NAFLD的治疗作用。NAFLD是通过给除对照组外的所有人喂食高脂高糖饮食16周而产生的。从第13周开始，PH组和DD组分别灌胃PH或DD水溶液，其余两组灌胃等量蒸馏水。末次给药后12小时，每组随机抽取8只大鼠。麻醉后，收集腹主动脉血和肝组织。观察肝组织形态学变化，并检测血清谷丙转氨酶（ALT）、谷草转氨酶（AST）、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）、高密度脂蛋白胆固醇（HDL-C）、空腹血糖（FBG）、转化生长因子-β1（TGF-β1）、肿瘤坏死因子-α（TNF-α）、Toll样受体4（TLR 4）、结果与对照组比较，模型组大鼠血清ALT、AST、TC、TG、LDL-C、FBG、TGF-β1、TNF-α、TLR 4、NF-κ B均显著升高（P< .01）。与模型组比较，PH组和DD组上述指标均显著降低（P<0.05）。结论DD对NAFLD大鼠的治疗效果与PH组相当，其作用包括降低肝脏脂肪变性、血脂和血糖水平。我们推测DD对高脂高糖饮食诱导的大鼠NAFLD有抑制作用。
ObjectiveTo establish an animal model consistent with the occurrence and development of non-alcoholic fatty liver disease (NAFLD) with which to assess the effects of a classical traditional Chinese medicine formula known asDachaihuDecoction (DD) on NAFLD.MethodsSixty rats were randomized into four groups: control, model, pioglitazone hydrochloride (PH) and DD in equal. NAFLD was produced via administration of a high-fat high-sugar diet for 16 weeks in all but the control group. From the 13th week, a solution of PH or DD prepared with water was delivered via intragastric administration to the PH and DD groups; the remaining two groups received an equivalent volume of distilled water. Twelve hours from the last administration, we selected eight rats from each group in random. After anesthetization, the abdominal aorta blood and liver tissues were collected. The morphological changes were observed and the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), fasting plasma glucose (FBG), transforming growth factor-β1 (TGF-β1), tumor necrosis factor-α (TNF-α), toll-like receptor-4 (TLR4), and nuclear factor-kappa B (NF-кB) were tested.ResultsCompared with the control group, the levels of serum ALT, AST, TC, TG, LDL-C and FBG, and TGF-β1, TNF-α, TLR4, NF-кB in the model group all showed significant increases (P< .01). Compared with the model group, these same indicators in the PH and DD groups all showed remarkable decreases (P< .05).ConclusionThe efficacy of DD in NAFLD rats was shown to be effectively equivalent to that of PH, with demonstrated effects of DD that included reductions in hepatic steatosis and serum and hepatic lipid levels, and lowered blood glucose levels. We deduce that DD has an inhibitory effect on NAFLD induced by a high-fat high-sugar diet in rats.