Immunoperoxidase detection of baculovirus antigens in insect cells.
Immunoperoxidase detection of baculovirus antigens in insect cells.
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昆虫细胞中杆状病毒抗原的免疫过氧化物酶检测。
作者：
M. Summers;L. Volkman;C. Hsieh
采用间接免疫过氧化物酶技术，研究了苜蓿银纹夜蛾核型多角体病毒（Autographa californica nuclear polyhedrosis virus，AcMNPV）感染TN-368-10和TN-368-13细胞的过程。抗血清对包膜核衣壳检测同源抗原在6至8小时后感染，这是约2小时前出现的细胞内和细胞外的感染性病毒。使用多角体蛋白抗血清的类似试验表明，多角体蛋白首先在感染后12小时合成，在感染性非封闭病毒出现后2至4小时。免疫过氧化物酶技术也适用于其他四个无脊椎动物细胞系接种后，与AcMNPV。最显著的结果是，90%的AcMNPV接种的家蚕5细胞产生包膜核衣壳抗原和感染性病毒，但只有1%或更少的细胞产生多角体蛋白。这种差异强调了对独立于多面体产生的NPV感染的测定的需要。
The sequence of events in the infection of TN-368-10 and TN-368-13 cells by Autographa californica nuclear polyhedrosis virus (AcMNPV) was investigated by using the indirect immunoperoxidase technique. Antisera raised against enveloped nucleocapsids detected homologous antigens at 6 to 8 h post infection which was about 2 h before the appearance of both intracellular and extracellular infectious virus. Similar tests using polyhedrin antiserum showed that polyhedrin is first synthesized at 12 h post infection, 2 to 4 h after the appearance of infectious nonoccluded virus. The immunoperoxidase technique was also applied to four other invertebrate cell lines after inoculation with AcMNPV. The most significant result was that 90% of AcMNPV-inoculated Bombyx mori 5 cells produced enveloped nucleocapsid antigens and infectious virus but only 1% or less of the cells produced polyhedrin. This disparity emphasizes the need for assays for NPV infection that are independent of polyhedron production.