β-Galactosidase-Based Colorimetric Paper Sensor for Determination of Heavy Metals
β-Galactosidase-Based Colorimetric Paper Sensor for Determination of Heavy Metals
复制标题
DOI：
10.1021/ac202290d
复制
发表时间：
2011-11-15
影响因子：
7.4
通讯作者：
Brennan, John D.
中科院分区：
文献类型：
--
作者：
Hossain, S. M. Zakir;Brennan, John D.
我们展示了一种使用固相生物活性纸上实验室传感器快速、选择性和灵敏地检测重金属的新方法，该传感器的入口印有溶胶-凝胶包埋的试剂，从而可以对 β-半乳糖苷酶 (B-GAL) 的酶活性进行比色可视化。生物活性纸检测能够在短短 10 分钟内检测出一系列重金属，无论是单独的还是混合物，检测限如下： Hg(II) = 0.001 ppm；银（I）= 0.002 ppm，铜（II）= 0.020 ppm；镉（II）= 0.020 ppm；铅（II）= 0.140 ppm； Cr(VI) = 0.150 ppm；镍 (II) = 0.230 ppm。基于纸的检测不受Na+或K+等无毒金属离子的干扰，可用于检测掺入自来水或湖水中的重金属，并提供与原子吸收获得的值一致的定量数据。通过将标准显色金属传感试剂合并到多重生物活性纸传感器中，可以识别混合物中的特定金属，尽管检测限比酶测定低得多。纸基传感器对于资源有限地区和发展中国家的痕量重金属快速现场筛查具有重要价值。
We demonstrate a novel approach for rapid, selective, and sensitive detection of heavy metals using a solid-phase bioactive lab-on-paper sensor that is inlet printed with sol-gel entrapped reagents to allow colorimetric visualization of the enzymatic activity of beta-galactosidase (B-GAL). The bioactive paper assay is able to detect a range of heavy metals, either alone or as mixtures, in as little as 10 min, with detection limits as follows: Hg(II) = 0.001 ppm; Ag(I) = 0.002 ppm, Cu(II) = 0.020 ppm; Cd(II) = 0.020 ppm; Pb(II) = 0.140 ppm; Cr(VI) = 0.150 ppm; Ni(II) = 0.230 ppm. The paper-based assay was immune to interferences from nontoxic metal ions such as Na+ or K+, could be used to detect heavy metals that were spiked into tap water or lake water, and provided quantitative data that was in agreement with values obtained by atomic absorption. With the incorporation of standard chromogenic metal sensing reagents into a multiplexed bioactive paper sensor, it was possible to identify specific metals in mixtures, albeit with much lower detection limits than were obtained with the enzymatic assay. The paper-based sensor should be valuable for rapid, on-site screening of trace levels of heavy metals in resource limited areas and developing countries.