Inhibitory effects of pine nodule extract and its component, SJ-2, on acetylcholine-induced catecholamine secretion and synthesis in bovine adrenal medullary cells
Inhibitory effects of pine nodule extract and its component, SJ-2, on acetylcholine-induced catecholamine secretion and synthesis in bovine adrenal medullary cells
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松根提取物及其成分SJ-2对乙酰胆碱诱导的牛肾上腺髓质细胞儿茶酚胺分泌和合成的抑制作用
DOI：
10.1016/j.jphs.2017.03.006
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发表时间：
2017
期刊：
影响因子：
3.5
通讯作者：
Yanagihara N
中科院分区：
文献类型：
--
作者：
2.Li X;Horishita T;Toyohira Y;Shao H;Bai J;Bo H;Song X;Ishikane S;Yoshinaga Y;Satoh N;Tsutsui M;Yanagihara N
油松（Pinus tabulaeformis）或马尾松（Pinus massoniana）的树皮在树木表面增殖形成的松结节（matsufushi）的提取物在中国传统医学中被用作关节疼痛、风湿、神经痛、痛经和其他病症的止痛剂。本文报道了松取物及其组分对培养的牛肾上腺髓质细胞分泌和合成儿茶酚胺的影响。我们发现，松芝提取物（0.0003-0.005%）及其组分SJ-2（5-羟基-3-甲氧基-反式-二苯乙烯）（0.3-100 μM）浓度依赖性地抑制乙酰胆碱（一种生理促分泌素）诱导的儿茶酚胺分泌，而其它三种则无此作用。松取物（0.0003-0.005%）和SJ-2（0.3-100 μM）对乙酰胆碱诱导的~（45）Ca ~（2+）内流也有浓度依赖性的抑制作用，与其对儿茶酚胺分泌的抑制作用相似。它们还抑制乙酰胆碱诱导的14 C-儿茶酚胺合成和酪氨酸羟化酶活性。在表达α3β4烟碱型乙酰胆碱受体的异种卵母细胞中，松下提取物（0.00003-0.001%）和SJ-2（1-100 μM）直接抑制乙酰胆碱诱发的电流。目前的研究结果表明，SJ-2，以及松下提取物，抑制乙酰胆碱诱导的儿茶酚胺分泌和合成的抑制烟碱乙酰胆碱受体离子通道在牛肾上腺髓质细胞。
Extract of pine nodules (matsufushi) formed by bark proliferation on the surface of trees ofPinus tabulaeformisorPinus massonianahas been used as an analgesic for joint pain, rheumatism, neuralgia, dysmenorrhea and other complaints in Chinese traditional medicine. Here we report the effects of matsufushi extract and its components on catecholamine secretion and synthesis in cultured bovine adrenal medullary cells. We found that matsufushi extract (0.0003–0.005%) and its component, SJ-2 (5-hydroxy-3-methoxy-trans-stilbene) (0.3–100 μM), but not the other three, concentration-dependently inhibited catecholamine secretion induced by acetylcholine, a physiological secretagogue. Matsufushi extract (0.0003–0.005%) and SJ-2 (0.3–100 μM) also inhibited45Ca2+influx induced by acetylcholine in a concentration-dependent manner, similar to its effect on catecholamine secretion. They also suppressed14C-catecholamine synthesis and tyrosine hydroxylase activity induced by acetylcholine. InXenopusoocytes expressing α3β4 nicotinic acetylcholine receptors, matsufushi extract (0.00003–0.001%) and SJ-2 (1–100 μM) directly inhibited the current evoked by acetylcholine. The present findings suggest that SJ-2, as well as matsufushi extract, inhibits acetylcholine-induced catecholamine secretion and synthesis by suppression of nicotinic acetylcholine receptor-ion channels in bovine adrenal medullary cells.