A comprehensive analysis of gene expression alterations in a newly synthesized Paspalum notatum autotetraploid
A comprehensive analysis of gene expression alterations in a newly synthesized Paspalum notatum autotetraploid
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DOI：
10.1016/j.plantsci.2005.03.015
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发表时间：
2005-07
期刊：
影响因子：
5.2
通讯作者：
L. Martelotto;J. P. Ortiz;J. Stein;F. Espinoza;C. Quarin;S. Pessino
中科院分区：
文献类型：
--
作者：
L. Martelotto;J. P. Ortiz;J. Stein;F. Espinoza;C. Quarin;S. Pessino
虽然多倍体中新变异的原因还不清楚，但它们可能涉及通过剂量调节、改变的调节相互作用和快速遗传/表观遗传变化引起的基因表达变化。这项工作的目的是有助于阐明在多倍体形成的早期阶段的基因调控的模式和机制。通过在二倍体基因型和新形成的同源四倍体衍生物的花上使用差异显示转录谱来监测100，000个基因的全基因组表达。通过反向Northern杂交验证了64个克隆的差异表达。序列分析表明，其中42个与26个已知功能的不同基因同源，而其余22个被归类为新序列。检测到的基因涉及DNA修复、染色质结构修饰、转录调控、蛋白质水解、蛋白质折叠、糖脂代谢和信号转导等过程。我们的研究结果表明，在百喜草多倍体化后立即发生基因表达改变的证据。
Although the causes of novel variation in polyploids are not well understood, they could involve changes in gene expression through dosage-regulation, altered regulatory interactions and rapid genetic/epigenetic changes. The objective of this work was to contribute to the elucidation of the patterns and mechanisms of gene regulation during the early stages of polyploid formation. The genome-wide expression of ∼10,000 genes was monitored by using differential display transcript profiling on flowers of a diploid genotype and a newly-formed autotetraploid derivative. Differential expression of 64 clones was validated by reverse-Northern blot. Sequence analysis showed that 42 were homologous to 26 distinct genes of known function while the remaining 22 were classified as novel sequences. Genes detected were involved in processes of DNA repair, chromatin structure modification, regulation of transcription, proteolysis, protein folding, carbohydrate and lipid metabolism and signal transduction. Our results show evidence for gene expression alterations occurring immediately after polyploidization in Paspalum.