Selection of DNA aptamers against polychlorinated biphenyls as potential biorecognition elements for environmental analysis
Selection of DNA aptamers against polychlorinated biphenyls as potential biorecognition elements for environmental analysis
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选择针对多氯联苯的 DNA 适体作为环境分析的潜在生物识别元件
DOI：
10.1016/j.ab.2012.01.026
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发表时间：
2012-04-15
影响因子：
2.9
通讯作者：
Wu, Lijun
中科院分区：
文献类型：
--
作者：
Xu, Shengmin;Yuan, Hang;Wu, Lijun
多氯联苯因其对人类健康的潜在毒性，几十年来一直受到人们的关注。为了高效、灵敏地追踪多氯联苯，人们开发了许多检测方法。适配子是一类新的诊断工具，被认为是检测污染物的额外候选者。在目前的研究中，我们报道了通过FluMag-SELEX(一种改进的SELEX[指数富集型配体的系统进化]技术)分离的DNA适配子，它识别解离常数(K-d值)到微摩尔范围的多氯联苯。利用所选择的适配子，利用金纳米粒子建立了高灵敏的基于适配子的荧光检测多氯联苯的方法，线性范围从0.1到100 ng/ml。此外，与几个常见的PCB77结构类似物相比，我们的基于适配子的金纳米探针对3，3‘，4，4’-四氯联苯(PCB77)表现出了特异性。这些结果为使用适配子作为生物识别元件进行简单快速的环境监测提供了可能性。(C)2012 Elsevier Inc.保留所有权利。
Polychlorinated biphenyls (PCBs) have been of major concerns for decades due to their potential toxicity to human health. To trace the PCBs efficiently and sensitively, many detection methods have been developed. Aptamers, a new class of diagnostic tools, are considered to be such additional candidates for detection of pollutants. In the current study, we report the DNA aptamers, isolated by FluMag-SELEX (a modified SELEX [systematic evolution of ligands by exponential enrichment] technology), that recognize PCBs with the dissociation constants (K-d values) down to the micromolar range. Using the selected aptamers, a highly sensitive aptamer-based fluorescent assay for detection of PCBs was established using gold nanoparticles, with a widely linear range from 0.1 to 100 ng/ml. Moreover, our aptamer-based gold nanoprobe displays specificity toward 3,3',4,4'-tetrachlorobiphenyl (PCB77) compared with a few common PCB77 structural analogs. These results open the possibility of using aptamers as biorecognition elements for easy and fast environmental monitoring. (C) 2012 Elsevier Inc. All rights reserved.