Expression of MIP-3α/CCL20, a macrophage inflammatory protein in oral squamous cell carcinoma
Expression of MIP-3α/CCL20, a macrophage inflammatory protein in oral squamous cell carcinoma
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DOI：
10.1016/s0003-9969(02)00167-x
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发表时间：
2003-02-01
影响因子：
3
通讯作者：
Kaku, T
中科院分区：
文献类型：
--
作者：
Abiko, Y;Nishimura, M;Kaku, T
我们在体内和体外检测了 MIP-3alpha/CCL20 在口腔鳞状细胞癌 (SCC) 中的表达。此外，我们还研究了MIP-3a/CCL20的表达是否受细菌调节。感染和炎症细胞因子。为了测定MIP-3α的mRNA水平，使用LightCycter TM 使用双链DNA染料SYBR Green 1进行定量逆转录聚合酶链式反应(RT-PCR)。口腔上皮细胞。细胞和六种 SCC 细胞系（SCC-9、SAS、BSC-OF、HSC-4、HSC、Ca9-22）被发现表达 MIP-3α mRNA。 MIP-3α的表达通过伴随放线放线杆菌的感染以及脂多糖和TNF-α的刺激而上调。通过原位杂交，SCC 中可检测到的 MIP-3α 表达主要位于与棘层相对应的上皮珠处。这些结果表明MIP-3α有助于细菌感染的口腔免疫反应，并可能参与鳞状细胞癌的生长。 (C) 2003 Elsevier Science Ltd. 保留所有权利。
We have examined the expression of MIP-3alpha/CCL20 in oral squamous cell carcinoma (SCC) in vivo and in vitro. In addition, we have investigated whether the expression of MIP-3a/CCL20 is regulated by bacterial. infection and inflammatory cytokines. In order to determine the mRNA level of MIP-3alpha, quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed with LightCycter(TM) using the double-stranded DNA dye, SYBR Green 1. Oral epithelial. cells and six SCC cell lines (SCC-9, SAS, BSC-OF, HSC-4, HSC, Ca9-22) were found to express MIP-3alpha mRNA. The expression of MIP-3alpha was upregutated by infection with Actinobacillus actinomycetemcomitans and by stimulation with lipopolysaccharide and TNF-alpha. By in situ hybridization, the detectable MIP-3alpha expression in SCC was localized primarily at the epithelial pearls corresponding to the spinous layer. These results suggest that MIP-3alpha contributes to the oral immunoresponse to bacterial infection, and may be involved in the growth of SCC. (C) 2003 Elsevier Science Ltd. All rights reserved.