Stochastic sensing of proteins with receptor-modified solid-state nanopores
Stochastic sensing of proteins with receptor-modified solid-state nanopores
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DOI：
10.1038/nnano.2012.24
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发表时间：
2012-04-01
影响因子：
38.3
通讯作者：
Rant, Ulrich
中科院分区：
文献类型：
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作者：
Wei, Ruoshan;Gatterdam, Volker;Rant, Ulrich
固态纳米孔能够对单个分子进行无标记分析。通过在纳米孔内固定一个分子受体来增加生化选择性是可能的，但是在这些修饰的纳米孔中很难保持单分子的敏感性。在这里，我们证明了用硝基三乙酸受体化学修饰的金属化氮化硅纳米孔可以用于蛋白质的随机传感。实时观察蛋白质与受体的可逆结合和解结合过程，并从单分子结合事件中统计分析相互作用参数。为了证明这种方法的多用途性，我们检测了his标记的蛋白质，并区分了啮齿动物IgG抗体的亚类。
Solid-state nanopores are capable of the label-free analysis of single molecules. It is possible to add biochemical selectivity by anchoring a molecular receptor inside the nanopore, but it is difficult to maintain single-molecule sensitivity in these modified nanopores. Here, we show that metallized silicon nitride nanopores chemically modified with nitrilotriacetic acid receptors can be used for the stochastic sensing of proteins. The reversible binding and unbinding of the proteins to the receptors is observed in real time, and the interaction parameters are statistically analysed from single-molecule binding events. To demonstrate the versatile nature of this approach, we detect His-tagged proteins and discriminate between the subclasses of rodent IgG antibodies.