Rapid identification of non-human sequences in high-throughput sequencing datasets
Rapid identification of non-human sequences in high-throughput sequencing datasets
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DOI：
10.1093/bioinformatics/bts100
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发表时间：
2012-04-15
期刊：
影响因子：
5.8
通讯作者：
Khavari, Paul A.
中科院分区：
文献类型：
--
作者：
Bhaduri, Aparna;Qu, Kun;Khavari, Paul A.
非人类序列快速识别 (RINS) 是一种基于交叉的病原体检测工作流程，利用用户提供的自定义参考基因组集来识别深度测序数据集中的非人类序列。在不到 2 小时内，RINS 正确识别了数据集 SRR73726 中的已知病毒，并且与任何能够运行先决条件比对和组装程序的计算机兼容。 RINS 准确识别数据集中完整或突变的非人类基因组的测序读数，并利用这些非人类序列可靠地生成重叠群（补充材料）。
Rapid identification of non-human sequences (RINS) is an intersection-based pathogen detection workflow that utilizes a user-provided custom reference genome set for identification of non-human sequences in deep sequencing datasets. In < 2h, RINS correctly identified the known virus in the dataset SRR73726 and is compatible with any computer capable of running the prerequisite alignment and assembly programs. RINS accurately identifies sequencing reads from intact or mutated non-human genomes in a dataset and robustly generates contigs with these non-human sequences (Supplementary Material).