Epidermal growth factor promotes rapid response to epithelial injury in rabbit duodenum in vitro.
Epidermal growth factor promotes rapid response to epithelial injury in rabbit duodenum in vitro.
复制标题
表皮生长因子在体外促进兔十二指肠上皮损伤的快速反应。
作者：
Martin Riegler;Roland Sedivy;T. Sogukoglu;E. Cosentini;G. Bischof;Béla Teleky;W. Feil;R. Schiessel;Gerhard Hamilton;Etienne Wenzl
背景与目标
生长因子主要参与肠道上皮屏障功能的调节。本研究探讨了表皮生长因子（EGF）和胰岛素样生长因子1（IGF-1）对离体兔十二指肠上皮重建的影响。
方法
将兔十二指肠粘膜条固定在Ussing室中，在腔中暴露于10 mmol/L HCl 10 min，然后与单独的缓冲液或含有不同浓度的EGF和IGF-1的腔缓冲液孵育3 h。电阻由电位差和短路电流计算。通过H& E染色切片上的形态测定法评估损伤。
结果
损伤后10分钟，HCl导致电阻从112 +/- 2降至51 +/- 4 ohms x cm 2（n = 6; P < 0.05）。损伤后用25或50 ng/mL管腔EGF治疗3小时刺激阻力分别恢复至94 +/- 3和104 +/- 3 Ω x cm 2，而对照组为81 +/- 3 Ω x cm 2（P < 0.05）。损伤后10分钟，62%的粘膜受损;损伤后3小时，25和50 ng/mL EGF组的损伤分别降低至24% +/- 1.09%和10% +/- 1.42%，对照组为38% +/- 0.93%（每组n = 6）。EGF刺激肠上皮细胞迁移。IGF-1不损害上皮恢复。
结论
EGF，而不是IGF-1，促进上皮重建的兔十二指肠在体外。
BACKGROUND & AIMS
Growth factors are mainly involved in the regulation of intestinal epithelial barrier function. This study investigated the effect of epidermal growth factor (EGF) and insulin-like growth factor 1 (IGF-1) on epithelial restitution of rabbit duodenum in vitro.
METHODS
Rabbit duodenal mucosal strips mounted in an Ussing chamber were luminally exposed to 10 mmol/L HCl for 10 minutes and then incubated with buffer alone or luminal buffer containing various concentrations of EGF and IGF-1 for 3 hours. Resistance was calculated from potential difference and short-circuit current. Damage was assessed by morphometry on H&E-stained sections.
RESULTS
HCl caused resistance to decrease from 112 +/- 2 to 51 +/- 4 ohms x cm2 10 minutes after injury (n = 6; P < 0.05). Postinjury treatment with 25 or 50 ng/mL luminal EGF for 3 hours stimulated resistance to recover to 94 +/- 3 and 104 +/- 3 ohms x cm2, respectively, vs. 81 +/- 3 omega x cm2 in controls (P < 0.05). Ten minutes after injury, 62% of the mucosa was damaged; 3 hours after injury, damage was reduced to 24% +/- 1.09% and 10% +/- 1.42% in the 25 and 50 ng/mL EGF group, respectively, vs. 38% +/- 0.93% in controls (n = 6 per group). EGF stimulated enterocyte migration. IGF-1 did not impair epithelial restitution.
CONCLUSIONS
EGF, but not IGF-1, promoted epithelial restitution of rabbit duodenum in vitro.
胰岛素样生长因子对 T84 细胞单层通透性的调节。
DOI：
10.1152/ajpcell.1992.262.1.c207
发表时间：
1992
期刊：
The American journal of physiology
影响因子：
--
作者：
McRoberts,JA;Riley,NE
通讯作者：
Riley,NE
在粘膜愈合的体外模型中，生长因子、基质蛋白和药物对肠上皮细胞迁移和增殖的独立调节。
影响因子：
3.8
作者：
Basson,MD;Modlin,IM;Flynn,SD;Jena,BP;Madri,JA
通讯作者：
Madri,JA