Dispersant affects the cellular influences of single-wall carbon nanotube: the role of CNT as carrier of dispersants
Dispersant affects the cellular influences of single-wall carbon nanotube: the role of CNT as carrier of dispersants
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DOI：
10.3109/15376516.2012.755595
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发表时间：
2013-06-01
影响因子：
3.2
通讯作者：
Fujita, Katsuhide
中科院分区：
文献类型：
--
作者：
Horie, Masanori;Stowe, Mayumi;Fujita, Katsuhide
碳纳米管(CNT)作为一种功能材料在工程和生命科学中的应用日益广泛。为了评价碳纳米管的体外细胞毒性，一些化学和生物试剂被用作分散剂。本研究考察了六种化学或生物试剂作为分散剂对细胞的影响。将Pluronic F-127、Pluronic F-68、1，2-二棕榈酰甘油-3-磷胆碱(DPPC)、肺表面活性物质表面活性物质(R)、牛血清白蛋白(BSA)和吐温80用于制备碳纳米管介质分散剂。各试剂对人肺癌A549细胞存活率的影响较小。然而，Pluronic F-127、DPPC、Surfacten(R)和Tween80诱导细胞内活性氧(ROS)水平升高。接下来，以每种试剂为分散剂，制备碳纳米管-介质分散体，并将其应用于A549细胞。细胞的影响取决于分散剂的种类。CNT分散体包括Pluronic(R)F-127、Surfacten(R)、DPPC和Tween80可使细胞向培养上清液中释放乳酸脱氢酶。在CNT分散体包括除BSA外的每个试剂的暴露细胞中观察到细胞内ROS水平的诱导。这些结果表明，吸附在碳纳米管表面的分散剂影响其细胞影响，特别是氧化应激的诱导。
The application of carbon nanotube (CNT) as a functional material to engineering and life sciences is advanced. In order to evaluate the cytotoxicity of CNT in vitro, some chemical and biological reagents are used for dispersants. In the present study, the cellular influences of six kinds of chemical or biological reagents used as dispersants were examined. Pluronic F-127, Pluronic F-68, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), pulmonary surfactant preparation Surfacten (R), bovine serum albumin (BSA) and Tween 80 were used in the preparation of CNT-medium dispersants. The influences of each reagent on cell viability in human lung carcinoma A549 cells were small. However, Pluronic F-127, DPPC, Surfacten (R) and Tween 80 induced an increase of intracellular reactive oxygen species (ROS) level. Next, CNT-medium dispersions were prepared, using each reagent as a dispersant and applied to A549 cells. The cellular influences depended on the kind of dispersant. Cells exposed to CNT dispersion including Pluronic (R) F-127, Surfacten (R), DPPC and Tween 80 showed LDH release to the culture supernatant. Induction of intracellular ROS level was observed in cells exposed to CNT dispersion including each reagent except BSA. These results suggest that the adsorbed dispersant reagents on the surface of the CNT affect its cellular influences, particularly the induction of oxidative stress.