The p38 MAPK inhibitor, SB203580, inhibits cell invasion by Neospora caninum
The p38 MAPK inhibitor, SB203580, inhibits cell invasion by Neospora caninum
复制标题
DOI：
10.1007/s00436-016-5346-1
复制
发表时间：
2017-02-01
影响因子：
2
通讯作者：
Li, Jianhua
中科院分区：
文献类型：
--
作者：
Jin, Xiaoxia;Gong, Pengtao;Li, Jianhua
顶复门寄生虫新孢子虫是一种专性细胞内寄生原生动物。它可以导致世界各地许多动物的严重疾病。感染犬新孢子虫会导致怀孕动物流产和新生儿神经肌肉疾病，给畜牧业造成巨大的经济损失。然而，犬新孢子虫侵袭细胞的机制仍不清楚。本文旨在研究 p38 MAPK 抑制剂 SB203580 对犬新孢子虫侵袭宿主细胞的影响。结果表明，犬新孢子虫中存在推定的 p38 MAPK 同源物，并且将犬新孢子虫与 SB203580 一起孵育可显着降低速殖子运动和微线体胞吐作用（NcMIC2、3 和 6）。此外，用SB203580处理或预处理MDBK细胞可有效减少犬新孢子虫的细胞侵袭。因此，SB203580 影响寄生虫和宿主细胞，从而抑制犬新孢子虫的细胞侵袭。
The Apicomplexan parasite Neospora caninum is an obligate intracellular parasitic protozoan. It can cause severe diseases in a number of animals throughout the world. Infection with N. caninum leads to abortions in pregnant animals and neuromuscular disorders of newborns which cause great economic losses to animal husbandry. However, the mechanism of cell invasion by N. caninum is still unclear. This paper aims to investigate the impact of SB203580, a p38 MAPK inhibitor, on host cell invasion by N. caninum. The results suggested the presence of putative p38 MAPK homologues in N. caninum, and incubation of N. caninum with SB203580 markedly reduced the tachyzoite motility and microneme exocytosis (NcMIC2, 3, and 6). Furthermore, treatment or pretreatment of MDBK cells with SB203580 effectively reduced cell invasion by N. caninum. Therefore, SB203580 affected both, parasites and host cells, resulting in inhibition of cell invasion by N. caninum.