Deciphering priming-induced improvement of rapeseed (Brassica napus L.) germination through an integrated transcriptomic and proteomic approach
Deciphering priming-induced improvement of rapeseed (Brassica napus L.) germination through an integrated transcriptomic and proteomic approach
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DOI：
10.1016/j.plantsci.2014.11.008
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发表时间：
2015-02-01
期刊：
影响因子：
5.2
通讯作者：
Quinet, Muriel
中科院分区：
文献类型：
--
作者：
Kubala, Szymon;Garnczarska, Malgorzata;Quinet, Muriel
用-1.2MPa聚乙二醇6000引发的油菜种子显示出改善的发芽性能。为了更好地理解启动对种子萌发的有益作用，使用全局表达谱分析方法首次比较启动过程（浸泡、干燥）、整个启动过程和随后萌发的关键阶段的启动种子的转录组学和蛋白质组学数据。以甘蓝型油菜（Brassica napus）为模型，研究了油菜种子萌发过程中的主要影响因素，包括952个基因和75个蛋白质。转录与翻译不协调，导致mRNA水平和蛋白质丰度之间的对应关系有限。引发种子的浸泡、干燥和最终萌发触发了不同的特异性途径，因为只有少数基因和蛋白质参与了种子引发的所有阶段，而绝大多数基因和蛋白质只参与了一个单一的阶段。特别注意的是基因和蛋白质参与转录，翻译，储备动员，水的吸收，细胞周期和氧化应激过程。(C)2014爱思唯尔爱尔兰有限公司版权所有。
Rape seeds primed with -1.2 MPa polyethylene glycol 6000 showed improved germination performance. To better understand the beneficial effect of osmopriming on seed germination, a global expression profiling method was used to compare, for the first time, transcriptomic and proteomic data for osmoprimed seeds at the crucial phases of priming procedure (soaking, drying), whole priming process and subsequent germination. Brassica napus was used here as a model to dissect the process of osmopriming into its essential components.A total number of 952 genes and 75 proteins were affected during the main phases of priming and post-priming germination. Transcription was not coordinately associated with translation resulting in a limited correspondence between mRNAs level and protein abundance. Soaking, drying and final germination of primed seeds triggered distinct specific pathways since only a minority of genes and proteins were involved in all phases of osmopriming while a vast majority was involved in only one single phase. A particular attention was paid to genes and proteins involved in the transcription, translation, reserve mobilization, water uptake, cell cycle and oxidative stress processes. (C) 2014 Elsevier Ireland Ltd. All rights reserved.