Vascular superoxide production by NAD(P)H oxidase - Association with endothelial dysfunction and clinical risk factors
Vascular superoxide production by NAD(P)H oxidase - Association with endothelial dysfunction and clinical risk factors
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DOI：
10.1161/01.res.86.9.e85
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发表时间：
2000-05-12
影响因子：
20.1
通讯作者：
Channon, KM
中科院分区：
文献类型：
--
作者：
Guzik, TJ;West, NEJ;Channon, KM
超氧阴离子在血管疾病状态中起着重要作用。在血管疾病的实验模型中，超氧化物歧化产物的增加导致一氧化氮(NO)生物活性降低和内皮功能障碍。我们测定了人体血管中NAD(P)H氧化酶产生的超氧化物歧化产物，并探讨了NAD(P)H氧化酶活性、NO介导的内皮功能与动脉粥样硬化的临床危险因素的关系。血管超氧化物的主要来源是一种依赖NAD(P)H的氧化酶。血管NAD(P)H氧化酶活性升高与NO介导的血管松弛减少有关，此外，内皮血管松弛减少和血管NAD(P)H氧化酶活性增加均与动脉粥样硬化的临床危险因素增加相关，糖尿病和高胆固醇血症独立地与NADH依赖的超氧化物产生增加相关。血管NAD(P)H氧化酶活性升高与内皮功能障碍和临床危险因素之间的关系表明，NAD(P)H氧化酶介导的超氧化物产生在人类动脉粥样硬化中起着重要作用。本文全文可在http://www.circresaha.org.上查阅
Superoxide anion plays important roles in vascular disease states. Increased superoxide production contributes to reduced nitric oxide (NO) bioactivity and endothelial dysfunction in experimental models of vascular disease. We measured superoxide production by NAD(P)H oxidase in human blood vessels and examined the relationships between NAD(P)H oxidase activity, NO-mediated endothelial function, and clinical risk factors for atherosclerosis, Endothelium-dependent vasorelaxations and direct measurements of vascular superoxide production were determined in human saphenous veins obtained from 133 patients with coronary artery disease and identified risk factors. The predominant source of vascular superoxide production was an NAD(P)H-dependent oxidase. Increased vascular NAD(P)H oxidase activity was associated with reduced NO-mediated vasorelaxation, Furthermore, reduced endothelial vasorelaxations and increased vascular NAD(P)H oxidase activity were both associated with increased clinical risk factors for atherosclerosis, Diabetes and hypercholesterolemia were independently associated with increased NADH-dependent superoxide production. The association of increased vascular NAD(P)H oxidase activity with endothelial dysfunction and with clinical risk factors suggests an important role for NAD(P)H oxidase-mediated superoxide production in human atherosclerosis. The full text of this article is available at http://www.circresaha.org.