Heifers express G-protein coupled receptor 153 in anterior pituitary gonadotrophs in stage-dependent manner.
Heifers express G-protein coupled receptor 153 in anterior pituitary gonadotrophs in stage-dependent manner.
复制标题
小母牛在垂体前叶促性腺激素中以阶段依赖性方式表达 G 蛋白偶联受体 153。
DOI：
10.1111/asj.12920
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发表时间：
2017
影响因子：
2
通讯作者：
and Kadokawa Hiroya.
中科院分区：
文献类型：
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作者：
Pandey Kiran;Kereilwe Onalenna;and Kadokawa Hiroya.
我们最近发现，孤儿G蛋白偶联受体（GPR）153在小母牛的垂体前叶（AP）中表达，这使我们推测GPR 153与促性腺激素释放激素受体（GnRHR）在促性腺激素细胞的质膜上共定位，并在生殖周期的特定时间表达。为了验证这一假设，我们通过免疫荧光显微镜检查了AP组织和培养的AP细胞中GnRHR、GPR 153和促黄体生成素或促卵泡激素的共表达。在促性腺激素细胞中检测到GPR 153，并与质膜中的GnRHR共定位。在培养的促性腺激素细胞的细胞质中也检测到GPR 153。真实的时间PCR和蛋白质印迹分析发现，与排卵前或黄体晚期相比，在黄体早期AP组织中的表达较低（P< 0.05）。GPR 153基因的5′-侧翼区含有雌激素的共有反应元件序列，但不含孕激素。这些数据表明，一些但不是所有的GPR 153与促性腺激素细胞质膜中的GnRHR共定位，并且其表达在牛AP中呈阶段依赖性变化。
We recently found that orphan G‐protein‐coupled receptor (GPR)153 is expressed in the anterior pituitary (AP) of heifers, leading us to speculate that GPR153 colocalizes with gonadotropin‐releasing hormone receptor (GnRHR) in the plasma membrane of gonadotrophs and is expressed at specific times of the reproductive cycle. To test this hypothesis, we examined the coexpression of GnRHR, GPR153, and either luteinizing hormone or follicle‐stimulating hormone in AP tissue and cultured AP cells by immunofluorescence microscopy. GPR153 was detected in the gonadotrophs, and was colocalized with GnRHR in the plasma membrane. GPR153 was also detected in the cytoplasm of cultured gonadotrophs. Real‐time PCR and western blot analyses found that expression was lower (P< 0.05) in AP tissues during early luteal phase as compared to pre‐ovulation or late luteal phases. The 5′‐flanking region of theGPR153gene contained a consensus response element sequence for estrogen, but not for progesterone. These data suggest that some, but not all GPR153 colocalizes with GnRHR in the plasma membrane of gonadotrophs, and its expression changes stage‐dependently in the bovine AP.