Epstein-Barr viral load as a marker of lymphoma in AIDS patients
Epstein-Barr viral load as a marker of lymphoma in AIDS patients
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DOI：
10.1002/jmv.20238
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发表时间：
2005-01-01
影响因子：
12.7
通讯作者：
Gulley, ML
中科院分区：
文献类型：
--
作者：
Fan, HX;Kim, SC;Gulley, ML
EB 病毒 (EBV) 与获得性免疫缺陷综合征 (AIDS) 淋巴瘤的发病机制有关，并且病毒 DNA 存在于大约一半受影响患者的恶性细胞内。我们研究了与机会性感染的艾滋病患者相比，艾滋病淋巴瘤患者血浆中 EBV 病毒载量升高的程度。研究人员对 61 名艾滋病患者进行了研究，其中 35 名患有淋巴瘤（24 名非霍奇金淋巴瘤、6 名霍奇金淋巴瘤和 5 名脑淋巴瘤）和 26 名患有各种机会性感染的患者。原位杂交显示 17/28 AIDS 淋巴瘤（61%）的恶性细胞中存在 EBV 编码 RNA (EBER) 表达。在来自 35 名淋巴瘤患者的 232 个系列血浆样本和来自 AIDS 对照的 128 个样本中，使用靶向 BamH1W 序列的 TaqMan 探针通过定量聚合酶链反应 (Q-PCR) 检测了 EBV 病毒载量。在所有 17 名 EBER 阳性艾滋病淋巴瘤患者的血浆中均检测到 EBV，病毒载量范围为每毫升 34 至 1,500,000 拷贝（中位数为 3,210）。淋巴瘤治疗开始后，病毒载量通常迅速下降，除了两名患有持续性肿瘤的患者外，病毒载量一直无法检测到。在 11 名淋巴瘤缺乏 EBER 表达的 AIDS 患者和 26 名无淋巴瘤的对照患者中，血浆中 EBV 水平通常较低或检测不到（范围分别为 0-1,995 和 0-2,409，中位数分别为 0 和 0）。 EBV 病毒载量与人类免疫缺陷病毒 (HIV) 载量或 CD4 计数之间没有关联。总之，EBV 病毒载量有望成为辅助诊断和治疗 EBV 相关淋巴瘤患者的工具。 (C) 2005Wiley-Liss, Inc.
Epstein-Barr virus (EBV) is implicated in the pathogenesis of acquired immunodeficiency syndrome (AIDS) lymphoma, and viral DNA is present within the malignant cells in about half of affected patients. We examined the extent to which EBV viral load is elevated in the plasma of AIDS lymphoma patients compared to AIDS patients with opportunistic infections. Sixty-one AIDS patients were studied including 35 with lymphoma (24 non-Hodgkin, six Hodgkin, and five brain lymphoma) and 26 with various opportunistic infections. In situ hybridization revealed EBV encoded RNA (EBER) expression in the malignant cells of 17/28 AIDS lymphomas (61%). In 232 serial plasma samples from 35 lymphoma patients and in 128 samples from AIDS controls, EBV viral load was assayed by quantitative-polymerase chain reaction (Q-PCR) using a TaqMan probe targeting the BamH1W sequence. EBV was detected in plasma from all 17 EBER-positive AIDS lymphoma patients, with viral loads ranging from 34 to 1,500,000 copies per ml (median 3,210). Viral load usually fell rapidly upon initiation of lymphoma therapy and remained undetectable except in two patients with persistent tumor. In 11 AIDS patients, whose lymphoma lacked EBER expression, and in 26 control patients without lymphoma, levels of EBV in plasma were usually low or undetectable (range 0-1,995 and 0-2,409, median 0 and 0, respectively). There was no association between EBV viral load and human immunodeficiency virus (HIV) load or CD4 count. In conclusion, EBV viral load shows promise as a tool to assist in diagnosis and management of EBV-related lymphoma patients. (C) 2005Wiley-Liss, Inc.