Temporary focal ischemia in the mouse: Technical aspects and patterns of Fluoro-Jade evident neurodegeneration
Temporary focal ischemia in the mouse: Technical aspects and patterns of Fluoro-Jade evident neurodegeneration
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DOI：
10.1016/j.brainres.2005.02.021
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发表时间：
2005-04-25
期刊：
影响因子：
2.9
通讯作者：
Pennypacker, KR
中科院分区：
文献类型：
--
作者：
Duckworth, EAM;Butler, TL;Pennypacker, KR
脑梗塞动物模型对于了解缺血性脑损伤后神经元存活的机制以及开发针对所有类型中风患者的治疗干预措施至关重要。啮齿动物已广泛用于此类研究。一种啮齿动物中风模型利用大脑中动脉（MCAO）的永久或暂时闭塞来产生缺血。自从 1989 年开发出血管内方法以来，MCAO 已普遍应用于大鼠，并且通常与 2,3,5-三苯基氯化四唑 (TTC) 染色配对用于每搏输出量测量。与此同时，基因改造小鼠能力的进步使得缺血性研究领域的研究取得了令人兴奋的进展。由于技术要求和生存问题，相对较少的实验室研究了小鼠的 MCAO 方法。我们目前的工作利用栓塞性中风的小鼠中脑闭塞（MCAO）模型来研究暂时性局灶性脑缺血后的神经元变性。 C57BI/6J 小鼠被用来使用 Fluoro-Jade 来检查 MCAO 的确切效果，Fluoro-Jade 是一种神经退行性疾病标记物，可以观察特定的大脑区域和注定死亡的细胞。建立了 MCAO 后神经元变性从 10 分钟到 7 天逐步升级的时间过程。讨论了这种适用于小鼠的短暂性局灶性缺血的流行方法的技术方面。 (c) 2005 Elsevier B.V. 保留所有权利。
Animal models of cerebral infarction are crucial to understanding the mechanisms of neuronal survival following ischemic brain injury and to the development of therapeutic interventions for victims of all types of stroke. Rodents have been used extensively in such research. One rodent model of stroke utilizes either permanent or temporary occlusion of the middle cerebral artery (MCAO) to produce ischemia. Since the development of an endovascular method for this was published in 1989, MCAO has been applied commonly to the rat, and often paired with 2, 3, 5-triphenyltetrazolium chloride (TTC) staining for stroke volume measurement. Meanwhile, advances in the ability to genetically alter mice have allowed exciting lines of research into ischemia. Because of technical demands and issues with survival, relatively few laboratories have investigated the MCAO method in the mouse. Our present work utilizes a mouse middle cerebral occlusion (MCAO) model of embolic stroke to study neuronal degeneration following temporary focal cerebral ischemia. C57BI/6J mice were used to examine the exact effects of MCAO using Fluoro-Jade, a marker of neurodegeneration that allows observation of specific brain regions and cells destined to die. A time course of escalating neuronal degeneration from 10 min to 7 days following MCAO was established. Technical aspects of this popular method for transient focal ischemia as it applies to the mouse are discussed. (c) 2005 Elsevier B.V. All rights reserved.