Eph/ephrin gradients in the retinotectal system of Rana pipiens: developmental and adult expression patterns.
Eph/ephrin gradients in the retinotectal system of Rana pipiens: developmental and adult expression patterns.
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林蛙视网膜顶盖系统中的 Eph/ephrin 梯度：发育和成体表达模式。
DOI：
10.1002/cne.21968
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发表时间：
2009
期刊：
影响因子：
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通讯作者：
Feldheim,DavidA
中科院分区：
文献类型：
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作者：
Scalia,Frank;Currie,JuliaR;Feldheim,DavidA
Eph/肝配蛋白受体/配体 A 和 B 家族在中枢神经系统发育过程中发挥着多种作用，包括形成视网膜顶盖投射的模式。然而，在中脑顶盖经历长期的前部到后部发育的物种中，它们的表达梯度与视网膜顶盖发育图和细胞发育梯度的一致性尚不清楚。通过使用解剖追踪方法和 3 H-胸苷神经元成像，我们将视网膜顶盖投影和顶盖细胞发育的时空进展映射到幼虫顶盖中的 Eph/ephrin 表达模式，并通过融合蛋白的原位亲和力分析进行研究。 EphA 表达在前顶盖（和颞部视网膜）中最大；肝配蛋白-A 的表达在后极（和鼻视网膜）最大。 EphB 表达在早期幼虫中分级，在后极部前面的后顶盖（以及腹侧视网膜）中表达量最大。 Tectal EphB 表达在后期变得均匀，并且在成人中保持如此，尽管其视网膜表达在腹侧保持最大。在早期幼虫中，EphA、EphB 和 ephrin-A 蛋白梯度彼此平行，并与视网膜投影的颞鼻轴对齐。早期 EphB 表达最大值与新有丝分裂后细胞的套层和细胞增殖的后部上皮区域之间的边界重叠，表明表达最大值与有丝分裂后细胞的初始迁移相关。 Ephrin-B 在所有年龄段的嗅球和背侧视网膜中均检测到表达，但在顶盖中未检测到。 J.Comp。内罗尔。 514:30–48, 2009。© 2009 Wiley-Liss, Inc.
Eph/ephrin‐receptor/ligand A and B families play a variety of roles during CNS development, including patterning the retinotectal projection. However, the alignment of their expression gradients with developing retinotectal maps and gradients of cellular development is not well understood in species whose midbrain tecta undergo a protracted anterior to posterior development. By using anatomical tracing methods and3H‐thymidine neuronography, we have mapped the retinotectal projection and the spatiotemporal progression of tectal cellular development onto Eph/ephrin expression patterns in the tectum of larvalRana pipiens, as studied by means of in situ affinity analysis with fusion proteins. EphA expression is maximal in anterior tectum (and temporal retina); ephrin‐A expression is maximal at the posterior pole (and nasal retina). EphB expression is graded in the early larva, where it is maximal in the posterior tectum just anterior to the posterior pole (and in the ventral retina). Tectal EphB expression becomes uniform at later stages and remains so in the adult, although its retinal expression remains maximal ventrally. In the early larva, EphA, EphB, and ephrin‐A protein gradients are parallel to each other and align with the temporonasal axis of the retinal projection. The early EphB expression maximum overlaps the boundary between the mantle layer of newly postmitotic cells and the posterior, epithelial region of cell proliferation, suggesting that the expression maximum is associated with the initial migrations of the postmitotic cells. Ephrin‐B expression was detected in the olfactory bulb and dorsal retina at all ages, but not in the tectum. J. Comp. Neurol. 514:30–48, 2009. © 2009 Wiley‐Liss, Inc.