DISTRIBUTION OF ANAEROBIC FUNGI IN THE DIGESTIVE-TRACT OF CATTLE AND THEIR SURVIVAL IN FECES
DISTRIBUTION OF ANAEROBIC FUNGI IN THE DIGESTIVE-TRACT OF CATTLE AND THEIR SURVIVAL IN FECES
复制标题
DOI：
10.1099/00221287-139-6-1395
复制
发表时间：
1993-06-01
期刊：
影响因子：
--
通讯作者：
TRINCI, APJ
中科院分区：
文献类型：
--
作者：
DAVIES, DR;THEODOROU, MK;TRINCI, APJ
最可能的数字程序被用来计数牛的消化液和粪便中的厌氧真菌种群。从瘤胃、瘤胃、皱胃、小肠、盲肠、大肠和粪便中分离到厌氧真菌。通过测定消化道每个器官中的消化量，可以估计牛体内厌氧真菌的总数，并对不同器官中的种群进行比较。除了对新收集的样本中的厌氧真菌进行计数外，还对在常温下干燥并在空气中储存长达9个月的消化液和粪便中的厌氧真菌进行了量化。这些实验表明，存在于后肠和粪便中的厌氧真菌比存在于胃和胃前器官中的厌氧真菌能够承受更高比例的干燥。我们的结果支持厌氧真菌的生活史由三个阶段组成的假设：游动游动孢子。营养叶状体和耐空气生存阶段(包囊或抗性游动孢子囊)。
A most probable numbers procedure was used to enumerate populations of anaerobic fungi in the digesta and faeces of cattle. Anaerobic fungi were isolated from the rumen, omasum, abomasum, small intestine, caecum, large intestine and faeces. By determining the amount of digesta in each organ of the digestive tract, it was possible to estimate the total population of anaerobic fungi in cattle and make comparisons between populations in different organs. In addition to enumerating anaerobic fungi in freshly collected samples, they were quantified in digesta and faeces which had been dried at ambient temperature and stored in air for up to 9 months. These experiments showed that a higher proportion of the anaerobic fungi present in the hindgut and faeces were able to withstand desiccation than those present within the gastric and pre-gastric organs. Our results support the hypothesis that the life cycle of anaerobic fungi consists of three stages; the motile zoospore. the vegetative thallus and an aero-tolerant survival stage (cyst or resistant zoosporangium).