ALKYL GLUCOSIDES AS EFFECTIVE SOLUBILIZING AGENTS FOR BOVINE RHODOPSIN - COMPARISON WITH SEVERAL COMMONLY USED DETERGENTS
ALKYL GLUCOSIDES AS EFFECTIVE SOLUBILIZING AGENTS FOR BOVINE RHODOPSIN - COMPARISON WITH SEVERAL COMMONLY USED DETERGENTS
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DOI：
10.1016/0005-2736(76)90428-4
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发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
LITMAN, BJ
中科院分区：
文献类型：
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作者：
STUBBS, GW;SMITH, HG;LITMAN, BJ
研究了辛基和癸基-β-D-葡萄糖苷作为增溶剂对牛视网膜杆外段盘膜的适用性，并与十六烷基三甲基溴化铵、N，N-二甲基十二烷基氧化胺、Emulphogene BC-720和毛地黄皂苷进行了比较。测量的性质包括视紫红质的热稳定性，通过添加11-顺式-视网膜的漂白视紫红质的再生性，和通过在紫外CD光谱中的变化测量的漂白视紫红质的变性率。通过观察洗涤剂对抹香鲸高铁肌红蛋白的吸收光谱和圆二色谱的影响，评价了洗涤剂的变性倾向。我们的结果表明，辛基葡萄糖苷是优于其他洗涤剂，与毛地黄皂苷可能例外，上述标准的上级。然而，与毛地黄皂苷不同，辛基葡糖苷提供椎间盘膜的快速增溶，并且本身是高度可溶的。癸基葡糖苷具有等同于或上级于辛基葡糖苷的性质，但盐和缓冲剂干扰其溶解盘膜的能力。烷基葡糖苷的明确的化学组成，易于通过透析去除，以及非变性特性使其成为膜研究的有吸引力的洗涤剂。
The suitability of octyl and decyl-β-d-glucoside as solubilizing agents for the bovine retinal rod outer segment disc membrane was investigated and compared to that of hexadecyltrimethylammonium bromide, N, N-dimethyldodecylamine oxide, Emulphogene BC-720 and digitonin. The properties measured included the thermal stability of rhodopsin, regenerability of bleached rhodopsin by addition of 11-cis-retinal, and the rate of denaturation of bleached rhodopsin as measured by changes in the ultraviolet CD spectrum. Denaturing tendencies of the detergents were also evaluated by observing their effects on the absorption and CD spectra of sperm whale metmyoglobin. Our results demonstrate that octyl glucoside is superior to the other detergents, with the possible exception of digitonin, by the above criteria. Unlike digitonin, however, octyl glucoside affords rapid solubilization of the disc membrane and is itself highly soluble. Decyl glucoside has properties equivalent or superior to octyl glucoside, but salts and buffers interfere with its ability to solubilize the disc membrane. The well defined chemical composition, ease of removal by dialysis, and non-denaturing properties of the alkyl glucosides make them attractive detergents for membrane research.