Construction of a flash-activated cyclic electron transport system by using bacterial reaction centers and the ubiquinone-cytochrome b-c1/c segment of mitochondria.
Construction of a flash-activated cyclic electron transport system by using bacterial reaction centers and the ubiquinone-cytochrome b-c1/c segment of mitochondria.
复制标题
利用细菌反应中心和线粒体的泛醌-细胞色素 b-c1/c 片段构建闪光激活的循环电子传递系统。
DOI：
10.1073/pnas.77.11.6339
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发表时间：
1980
影响因子：
11.1
通讯作者：
Dutton,PL
中科院分区：
文献类型：
--
作者：
Packham,NK;Tiede,DM;Mueller,P;Dutton,PL
单营业额内的线粒体复合物III的电子转移进行了研究相结合，在解决方案中，从牛的心脏与洗涤剂溶解的反应中心的Rhodoptera sphaeroides分离的复合物。通过短闪光激活引发电子转移导致细胞色素c的迅速氧化和细胞色素B的还原。随后观察到铁细胞色素c的还原伴随着铁细胞色素B的氧化，这两个反应都被添加放线菌素A抑制。通过复合物III的电子转移速率取决于周围的氧化还原电位，其方式与氧化还原组分的存在一致，推测类似于光合泛醌Qz，其是细胞色素B和c之间电子转移的强制性中间体。这些结果表明，在线粒体复合物III，细胞色素c，和光化学反应中心的组装循环电子转移。
Single-turnover electron transfer within the mitochondrial complex III has been studied by combining, in solution, the isolated complex from bovine heart with detergent-solubilized reaction centers of Rhodopseudomonas sphaeroides. Initiation of electron transfer by short flash activation resulted in the prompt oxidation of cytochrome c and reduction of cytochrome b. The subsequent reduction of ferricytochrome c was observed to be concomitant with the oxidation of the ferrocytochrome b, both reactions being inhibited by the addition of actimycin A. The rate of electron transfer through complex III is dependent upon the ambient redox potential poise in a way that is consistent with the presence of a redox component, presumably analogous to the photosynthetic ubiquinone Qz, which is an obligatory intermediate in electron transfer between cytochromes b and c. These results demonstrate cyclic electron transfer in a constructed assembly of mitochondrial complex III, cytochrome c, and photochemical reaction centers.