A major yellow-seed QTL on chromosome A09 significantly increases the oil content and reduces the fiber content of seed in Brassica napus
A major yellow-seed QTL on chromosome A09 significantly increases the oil content and reduces the fiber content of seed in Brassica napus
复制标题
A09染色体上的一个主要黄种子QTL显着增加了甘蓝型油菜种子的含油量并降低了纤维含量
DOI：
10.1007/s00122-022-04031-0
复制
发表时间：
2022-01-27
影响因子：
5.4
通讯作者：
Li, Maoteng
中科院分区：
文献类型：
--
作者：
Chao, Hongbo;Guo, Liangxing;Li, Maoteng
位于染色体A09上的一个黄籽主效QTL显著提高了甘蓝型油菜种子的含油量，降低了纤维含量。黄籽性状一直是油菜育种的主要目标，因为黄籽B。与黑籽B相比，欧洲油菜通常含有更高的油含量、更少的色素和多酚以及更低的纤维含量。napus，尽管控制这种相关性的机制仍不清楚。本研究以黄籽小麦B杂交获得的KN双单倍体群体为基础，对YST进行了QTL定位。油菜N53-2含油量较高，黑籽垦C8含油量较低。共检测到10个QTL，其中4个QTL在多个环境中稳定存在。利用QTL定位和BSR-Seq技术在A09染色体上定位到一个主效QTL cqSC-A09，该QTL对表型变异的解释率超过41%。YST的主效QTL cqSC-A09不仅控制种子颜色，而且影响种子含油量和纤维含量。更重要的是，该优势等位基因可以在提高油分含量的同时，降低色素和纤维含量。这是首次报道的同时控制籽粒颜色、含油量和纤维含量且效应较大的QTL，对选育高油、高品质的品种具有重要的应用价值。重要的候选基因，包括BnaA 09。JAZ1、BnaA09。GH3.3和BnaA 09。结合序列变异注释、表达差异和相互作用网络，鉴定了cqSC-A09的LOX 3，为今后进一步克隆和育种应用奠定了基础。
Key message A major yellow-seed QTL on chromosome A09 significantly increases the oil content and reduces the fiber content of seed in Brassica napus. The yellow-seed trait (YST) has always been a main breeding objective for rapeseed because yellow-seeded B. napus generally contains higher oil contents, fewer pigments and polyphenols and lower fiber content than black-seeded B. napus, although the mechanism controlling this correlation remains unclear. In this study, QTL mapping was implemented for YST based on a KN double haploid population derived from the hybridization of yellow-seeded B. napus N53-2 with a high oil content and black-seeded Ken-C8 with a relatively low oil content. Ten QTLs were identified, including four stable QTLs that could be detected in multiple environments. A major QTL, cqSC-A09, on chromosome A09 was identified by both QTL mapping and BSR-Seq technology, and explained more than 41% of the phenotypic variance. The major QTL cqSC-A09 for YST not only controls the seed color but also affects the oil and fiber contents in seeds. More importantly, the advantageous allele could increase the oil content and reduce the pigment and fiber content at the same time. This is the first QTL reported to control seed color, oil content and fiber content simultaneously with a large effect and has great application value for breeding high oil varieties with high seed quality. Important candidate genes, including BnaA09. JAZ1, BnaA09. GH3.3 and BnaA09. LOX3, were identified for cqSC-A09 by combining sequence variation annotation, expression differences and an interaction network, which lays a foundation for further cloning and breeding applications in the future.