Biosafe alternative to xylene: A comparative study.
Biosafe alternative to xylene: A comparative study.
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DOI：
10.4103/0973-029x.125199
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发表时间：
2013-09-01
期刊：
影响因子：
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通讯作者：
Sachdeva, Alisha
中科院分区：
文献类型：
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作者：
Negi, Amita;Puri, Abhiney;Sachdeva, Alisha
背景：二甲苯是组织学实验室中不可替代的化学品之一。然而，它被认为具有许多毒性作用。二甲苯的毒性作用包括心脏和肾脏损伤，一些致命的血液恶液质和其他不太危险的问题，如皮肤红斑，干燥，鳞屑和继发性感染。二甲苯的暴露和处理在脱蜡组织切片过程中是最大的。目的：本研究的目的是评估1.7%洗碗皂（DWS）溶液作为苏木精和伊红（H和E）染色脱蜡剂的功效，并将其与二甲苯进行比较。材料和方法：从30个福尔马林固定石蜡包埋（FFPE）组织中获得60个4 μ m切片，并分为两个不同的组，A组和B组。A组常规H、E染色，B组用1.7%DWS脱蜡液染色，统计学分析：Wilcoxon配对符号秩和检验（P值显著性≤0.05）。结果和结论：1.7%DWS是一种有效的替代二甲苯的脱蜡剂，同时也是一种生物危害较小、经济和快速的脱蜡剂。
BACKGROUND: Xylene in one of the non-substitutable chemical used in histology laboratories. However, it is known to have many toxic effects. The toxic effects of xylene include heart and kidney injuries, some fatal blood dyscrasia and other less dangerous problems, such as skin erythema, drying, scaling and secondary infections. The exposure and handling of xylene is maximum during deparaffinizing tissue sections.AIMS: The aim of this study was to evaluate the efficacy of 1.7% dishwashing soap (DWS) solution as a deparaffinizing agent for hematoxylin and eosin (H and E) staining and compare it with xylene.MATERIALS AND METHODS: Sixty sections of 4 mum were obtained from 30 formalin-fixed paraffin-embedded (FFPE) tissues and were considered in two different groups, groups A and B. Slides in group A were stained with routine H and E staining procedure; whereas, slides in group B were stained using 1.7% DWS as a deparaffinizing agent.STATISTICAL ANALYSIS USED: Wilcoxon matched-pairs signed rank test was used to calculate the test of significance (P-value significant at ≤0.05).RESULTS AND CONCLUSION: 1.7% DWS was found to be an effective alternative deparaffinizing agent to xylene and meanwhile facilitating as less biohazardous, economical and a faster deparaffinizing agent.