Regional differences in vulnerability of the cerebellar foliations of rats exposed to neonatal X-irradiation
Regional differences in vulnerability of the cerebellar foliations of rats exposed to neonatal X-irradiation
复制标题
暴露于新生X射线照射的大鼠小脑叶脆弱性的区域差异
DOI：
10.1016/j.neulet.2006.03.040
复制
发表时间：
2006-07-10
影响因子：
2.5
通讯作者：
Takeuchi, Yoshiki
中科院分区：
文献类型：
--
作者：
Li, Hong-Peng;Miki, Takanori;Takeuchi, Yoshiki
本研究的目的是阐明X射线照射大鼠小脑叶的易损性的地区差异。分别用钙结合蛋白-D28k(CB)和胶质纤维酸性蛋白(GFAP)免疫组织化学方法观察X射线照射对蒲肯野细胞和Bergmann神经胶质纤维组织学改变的影响。Wistar大鼠于出生后第1天(PND)接受1.5GyX射线照射，3周龄时取小脑进行组织学检查。与对照组相比，X射线照射组大鼠的小脑叶变小、异常。在叶I-III和VIA-VII中，由于与邻近的叶融合而观察到的小脑叶变较少。此外，这种异常的程度在后一种叶中更为严重。CB免疫反应(IR)浦肯野细胞表现为侵入颗粒层或白质的细小、短小、方向混乱的树突。另一方面，GFAP-IR Bergmann神经胶质纤维的突起没有延伸到与硬膜表面垂直的分子层，而且看起来很薄，没有方向性。相应地，叶I-III、VIA-VII和X的上述小脑异常比其他区域更严重。与这些叶的组织学变化相反，在X-射线照射和对照之间，叶IV-V没有明显的质量差异。这些发现表明，小脑叶对X射线照射的易感性存在地区差异。这种差异可能归因于小脑神经遗传梯度。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
The purpose of the present study was to elucidate regional differences in the vulnerability of cerebellar foliations of rats exposed to X-irradiation. Effects of X-irradiation on foliations were examined with respect to histological changes in Purkinje cells and Bergmann glial fibers by calbindin-D28k (CB) and glial fibrillary acidic protein (GFAP) immunohistochemistry, respectively. Wistar rats were exposed to X-irradiation (1.5 Gy) on postnatal day (PND) 1. At 3 weeks of age, the cerebellum was examined. The cerebella of rats exposed to X-irradiation showed smaller and abnormal foliations compared with controls. Fewer cerebellar foliations due to fusion with neighboring folia were observed in folia I-III and VIa-VII. Moreover, the extent of such abnormalities was more severe in the latter folia. CB-immunoreactive (IR) Purkinje cells exhibited thin, short, disoriented dendrites that had invaded the granular layer or white matter. On the other hand, GFAP-IR Bergmann glial fibers had not extended their processes into the molecular layer perpendicular to the pial surface, and they appeared thin and disoriented. Accordingly, the above cerebellar abnormalities were more severe in folia I-III, VIa-VII and X than in other regions. In contrast to the histological alterations in these folia, there were no apparent qualitative differences in folia IV-V between X-irradiated and controls. These findings indicate regional difference in the vulnerability of cerebellar folia to X-irradiation. Such differences might be attributed to the cerebellar neurogenetic gradient. (c) 2006 Elsevier Ireland Ltd. All rights reserved.