Detection of group A Streptococcus in tonsils from pediatric patients reveals high rate of asymptomatic streptococcal carriage.
Detection of group A Streptococcus in tonsils from pediatric patients reveals high rate of asymptomatic streptococcal carriage.
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DOI：
10.1186/1471-2431-12-3
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发表时间：
2012-01-09
期刊：
影响因子：
2.4
通讯作者：
Reid SD
中科院分区：
文献类型：
--
作者：
Roberts AL;Connolly KL;Kirse DJ;Evans AK;Poehling KA;Peters TR;Reid SD
A组链球菌（GAS）可引起儿童急性扁桃体咽炎，约20%的儿童是GAS的慢性携带者。先前已证明抗菌治疗不足以清除GAS运载。细菌生物膜是一种包裹在细胞外聚合物基质中的表面附着的细菌群落。生物膜已被证明可以提供针对免疫反应和抗生素治疗的保护性生态位，并且通常与复发性或慢性细菌感染有关。本研究的目的是检验扁桃体切除术时扁桃体组织内存在GAS的假设。采用盲法免疫荧光和组织学方法评价因扁桃体肥大或复发性GAS扁桃体咽炎而接受常规扁桃体切除术的儿童的腭扁桃体。在因扁桃体肥大而行扁桃体切除术的11/30（37%）儿童和因复发性GAS咽炎而行扁桃体切除术的10/30（33%）儿童中，使用抗GAS抗体的免疫荧光分析呈阳性。用抗GAS和抗细胞角蛋白8和18抗体的荧光显微镜显示GAS定位于扁桃体网状隐窝。扫描电子显微镜确定了三维社区的球菌相似的大小和形态GAS。这些群落的特征与体内动物模型的GAS生物膜相似。我们的研究显示，在维克森林医学院因扁桃体肥大或复发性GAS扁桃体咽炎而接受扁桃体切除术的儿童中，约三分之一的扁桃体网状隐窝内存在GAS。收集的组织通常是丢弃的组织，未收集患者标识符。因此，没有受试者正式入组。
Group A Streptococcus (GAS) causes acute tonsillopharyngitis in children, and approximately 20% of this population are chronic carriers of GAS. Antibacterial therapy has previously been shown to be insufficient at clearing GAS carriage. Bacterial biofilms are a surface-attached bacterial community that is encased in a matrix of extracellular polymeric substances. Biofilms have been shown to provide a protective niche against the immune response and antibiotic treatments, and are often associated with recurrent or chronic bacterial infections. The objective of this study was to test the hypothesis that GAS is present within tonsil tissue at the time of tonsillectomy. Blinded immunofluorescent and histological methods were employed to evaluate palatine tonsils from children undergoing routine tonsillectomy for adenotonsillar hypertrophy or recurrent GAS tonsillopharyngitis. Immunofluorescence analysis using anti-GAS antibody was positive in 11/30 (37%) children who had tonsillectomy for adenotonsillar hypertrophy and in 10/30 (33%) children who had tonsillectomy for recurrent GAS pharyngitis. Fluorescent microscopy with anti-GAS and anti-cytokeratin 8 & 18 antibodies revealed GAS was localized to the tonsillar reticulated crypts. Scanning electron microscopy identified 3-dimensional communities of cocci similar in size and morphology to GAS. The characteristics of these communities are similar to GAS biofilms from in vivo animal models. Our study revealed the presence of GAS within the tonsillar reticulated crypts of approximately one-third of children who underwent tonsillectomy for either adenotonsillar hypertrophy or recurrent GAS tonsillopharyngitis at the Wake Forest School of Medicine. The tissue collected was normally discarded tissue and no patient identifiers were collected. Thus, no subjects were formally enrolled.
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影响因子：
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