Identification and detoxification of glycolaldehyde, an unattended bioethanol fermentation inhibitor
Identification and detoxification of glycolaldehyde, an unattended bioethanol fermentation inhibitor
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DOI：
10.3109/07388551.2015.1128877
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发表时间：
2017-02
影响因子：
9
通讯作者：
L. Jayakody;J. Ferdouse;N. Hayashi;H. Kitagaki
中科院分区：
文献类型：
--
作者：
L. Jayakody;J. Ferdouse;N. Hayashi;H. Kitagaki
虽然已经有大约60种化合物被确定为木质纤维素水解物的有效发酵抑制剂，但我们的研究小组最近发现乙醇醛是第二代生物燃料生产中的关键发酵抑制剂。因此，我们开发了一种对乙醇醛具有耐受性的酿酒酵母菌株。在这项乙醇醛研究中，我们建立了新的方法来合理地改造耐抑制剂酿酒酵母菌株，包括工程氧化还原辅助因子和工程SUMOylation途径。这些新的技术维度为酿酒酵母的工程菌株克服木质纤维素乙醇生产工业化的关键障碍提供了一个新的平台。因此，本综述讨论了乙醇醛在生物燃料工业背景下的新的生化见解。
Abstract Although there have been approximately 60 chemical compounds identified as potent fermentation inhibitors in lignocellulose hydrolysate, our research group recently discovered glycolaldehyde as a key fermentation inhibitor during second generation biofuel production. Accordingly, we have developed a yeast S. cerevisiae strain exhibiting tolerance to glycolaldehyde. During this glycolaldehyde study, we established novel approaches for rational engineering of inhibitor-tolerant S. cerevisiae strains, including engineering redox cofactors and engineering the SUMOylation pathway. These new technical dimensions provide a novel platform for engineering S. cerevisiae strains to overcome one of the key barriers for industrialization of lignocellulosic ethanol production. As such, this review discusses novel biochemical insight of glycolaldehyde in the context of the biofuel industry.