THE INSECT TRACHEAL SYSTEM - A CONDUIT FOR THE SYSTEMIC SPREAD OF AUTOGRAPHA-CALIFORNICA-M NUCLEAR POLYHEDROSIS-VIRUS
THE INSECT TRACHEAL SYSTEM - A CONDUIT FOR THE SYSTEMIC SPREAD OF AUTOGRAPHA-CALIFORNICA-M NUCLEAR POLYHEDROSIS-VIRUS
复制标题
DOI：
10.1073/pnas.91.8.3224
复制
发表时间：
1994-04-12
影响因子：
11.1
通讯作者：
VOLKMAN, LE
中科院分区：
文献类型：
--
作者：
ENGELHARD, EK;KAMMORGAN, LNW;VOLKMAN, LE
杆状病毒在易感昆虫宿主体内建立全身性感染，即使宿主组织被基底层包围，细胞外基质排除了比这些病毒小的颗粒。利用重组加州签名虫M型核多角体病毒，在组成型启动子的控制下，含有lacZ报告基因，我们跟踪了ni毛虫幼虫的感染进展。我们发现，幼虫的气管系统（而不是之前认为的血细胞）的感染为这种病毒通过基底层并在宿主中传播提供了主要通道。气管表皮细胞，唯一已知的气管系统的细胞成分，共享一个共同的淋巴系统。局部这些细胞通过交叉的细胞质延伸相互接触，称为表皮足。气管系统的这两个特征很可能促进病毒的快速全身传播。本文的研究结果对昆虫病理学和生物防治领域具有重要的指导意义，并引起人们对寄主范围因素的重视。
Baculoviruses establish systemic infections within susceptible insect hosts, even though host tissues are surrounded by basal laminae, extracellular matrices that exclude particles smaller than these viruses. Using a recombinant Autographa californica M nuclear polyhedrosis virus containing a lacZ reporter gene under the control of a constitutive promoter, we followed the progression of infection in Trichoplusia ni larvae. We discovered that infection of the larval insect tracheal system (and not hemocytes, as thought previously) provides the major conduit for this virus to pass through basal laminae and to spread throughout the host. Tracheal epidermal cells, the only known cellular components of the tracheal system, share a common lymph system. Locally these cells contact one another by interdigitating cytoplasmic extensions called epidermal feet. These two features ot the tracheal system are likely to facilitate the rapid systemic spread of the virus. The findings reported here have major implications for the fields of insect pathology and biological control and usher in an important consideration regarding host-range factors.