A novel multiple-marker method for the early diagnosis of oral squamous cell carcinoma
A novel multiple-marker method for the early diagnosis of oral squamous cell carcinoma
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用于早期诊断口腔鳞状细胞癌的新型多标记方法
DOI：
10.1155/2009/510124
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Nkenke, Emeka
中科院分区：
文献类型：
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作者：
Ries, Jutta;Mollaoglu, Nur;Nkenke, Emeka
目的：黑色素瘤相关抗原-A(MAGE-A)的表达具有高度的癌细胞特异性。因此，它们可能是诊断恶性肿瘤的最合适的靶点。本研究旨在评价MAGE-A多重表达分析在口腔鳞状细胞癌(OSCC)诊断中的敏感性。方法：采用高灵敏度逆转录聚合酶链式反应(RT-PCR)方法检测70例口腔鳞状细胞癌和20例正常口腔黏膜(NOM)标本中12种MAGE-A亚型中10种单抗的表达。结果：MAGE-A在口腔鳞癌中的表达仅限于口腔鳞癌。单一抗原表达频率在10%~55%之间。然而，随着检测基因数量的增加，表达效率提高到93%。MAGE-A的表达与肿瘤的恶性程度显著相关(p=0.0001)。此外，多项MAGE-A检测还与口腔鳞癌的淋巴结转移发生率、分级及临床分期有关。结论：多项MAGE-A检测对口腔鳞癌的诊断价值较单一MAGE-A更敏感。即使在口腔鳞癌的早期阶段，多项MAGE-A表达分析也可能是一种非常敏感的诊断方法。
Objective: Melanoma associated antigens-A (MAGE-A) expression is highly specific to cancer cells. Thus, they can be the most suitable targets for the diagnosis of malignancy. The aim of this study was to evaluate the sensitivity of multiple MAGE-A expression analysis for the diagnosis of oral squamous cell carcinoma (OSCC).Methods: Total of 70 OSSC and 20 normal oral mucosal (NOM) samples of otherwise healthy volunteers were examined for the expression of 10 different single antigens out of 12 different MAGE-A subtypes by highly sensitive reverse transcriptase polymerase chain reaction (RT-PCR) methods. The results were correlated to clinicopathological parameters of tumor samples.Results: Expression of MAGE-A was restricted to OSCC. The expression frequency of single antigen was between 10% and 55%. However, expression rate was increased up to 93% by the elevated number of genes examined. A significant correlation was found between the expression of MAGE-A and malignancy (p = 0.0001). In addition, multiple MAGE-A detection has also correlated to the incidence of lymph node metastasis, grading and advanced clinical stages.Conclusions: Analysis of multiple MAGE-A expression is more sensitive than the analysis of a single MAGE-A for the diagnostic evaluation of OSCC. Multiple MAGE-A expression analysis may be a very sensitive method to be used for the diagnosis even in the early stage of OSCC.