Influence of on and other modifying factors on the distribution behavior of 4-quinolones to solid phases and humic acids studied by "negligible-depletion" SPME-HPLC
Influence of on and other modifying factors on the distribution behavior of 4-quinolones to solid phases and humic acids studied by "negligible-depletion" SPME-HPLC
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DOI：
10.1021/es999917y
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发表时间：
2000-12-01
影响因子：
11.4
通讯作者：
Hermens, JLM
中科院分区：
文献类型：
--
作者：
Lützhoft, HCH;Vaes, WHJ;Hermens, JLM
氟喹酮、恶喹酸和沙拉沙星是用于养鱼的4-喹诺酮类抗菌药物。本研究的目的是研究pH对这些化合物在缓冲溶液和Aldrich腐植酸(DOC)之间的分配行为的影响。采用可忽略耗尽的固相微萃取-高效液相色谱联用技术(ND-SPME-HPLC法)测定抗菌药物的游离浓度。结果表明，抗菌剂在缓冲液和固相微萃取纤维之间的分布可以描述为pH和电离常数的函数。建立了缓冲液和DDC之间的分配系数(D-DOC)。用pH和电离常数描述这种分布是不可能的。尽管氟喹诺酮类化合物的疏水性很低(K-OW<；10(1.7))，但D-DOC值的大小(10(3.4)-10(5.2))与高度疏水的化学品的D-DOC值的大小相同。
Flumequine, oxolinic acid, and sarafloxacin are 4-quinolone antimiciobials that are used in fish farming. The objective of this investigation was to study the influence of pH on the distribution behavior between buffer solutions and Aldrich humic acids (DOC) of these compounds. Negligible-depletion solid-phase microextraction coupled to highperformance liquid chromatography (nd-SPME-HPLC) was used to quantify the free concentration of the antimicrobials. It was shown that the distribution of the antimicrobials between buffer and the SPME fiber could be described as a function of pH and ionization constants. Distribution coefficients between buffer and DDC(D-DOC)were established. It was not possible to describe this distribution using pH and ionization constants. The magnitude of the D-DOC values (10(3.4)-10(5.2)) was found in the same range as for highly hydrophobic chemicals despite the low hydrophobicity of the F-quinolones (K-OW < 10(1.7)).