Disruption of the Golgi apparatus by brefeldin A inhibits the cytotoxicity of ricin, modeccin, and Pseudomonas toxin.
Disruption of the Golgi apparatus by brefeldin A inhibits the cytotoxicity of ricin, modeccin, and Pseudomonas toxin.
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DOI：
10.1016/0014-4827(91)90056-z
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发表时间：
1991-02
影响因子：
3.7
通讯作者：
T. Yoshida;C. Chen;M. Zhang;H. C. Wu
中科院分区：
文献类型：
--
作者：
T. Yoshida;C. Chen;M. Zhang;H. C. Wu
我们研究了蓖麻毒素在灯盏花素A(BFA)处理的细胞中的细胞毒性，灯盏花素A(BFA)极大地破坏了高尔基体的结构，导致高尔基体内容物和膜重新分布到内质网。BFA可抑制蓖麻毒素对中国仓鼠卵巢、正常大鼠肾脏和Vero细胞的细胞毒性，并可消除NH4Cl、黑素、苦马豆素、衣霉素或胞体酸化突变对蓖麻毒素细胞毒性的增强作用。BFA对莫德霉素和假单胞菌毒素的细胞毒性有保护作用，但对白喉毒素中毒无影响。在没有BFA的情况下，BFA的预处理不能保护细胞免受蓖麻毒素的影响。我们的结果表明，蓖麻毒素、莫德霉素和假单胞菌毒素具有共同的细胞内转运途径，从内体到高尔基体，在高尔基体中它们被释放到细胞质。相反，BFA缺乏对Vero细胞白喉毒素的保护，这表明白喉毒素是从酸化的内体中释放出来的，而不涉及高尔基体区域。
We have studied the cytotoxicity of ricin in cells treated with brefeldin A (BFA), which dramatically disrupts the structure of the Golgi apparatus causing Golgi content and membrane to redistribute to the ER. BFA inhibits the cytotoxicity of ricin in Chinese hamster ovary, normal rat kidney, and Vero cells and abolishes the enhancement of ricin cytotoxicity by NH4Cl, nigericin, swainsonine, and tunicamycin or by a mutation in endosomal acidification. BFA protects cells from the cytotoxicities of modeccin andPseudomonastoxin, but has no effect on the intoxication by diphtheria toxin. Pretreatment of BFA does not protect cells from ricin treatment in the absence of BFA. Our results suggest that ricin, modeccin, andPseudomonastoxin share a common pathway of intracellular transport from endosomes to the Golgi region where they are released into the cytosol. In contrast, the lack of protection of Vero cells from diphtheria toxin by BFA indicates that diphtheria toxin is released from acidified endosomes without involving the Golgi region.