QUEST FOR HUMAN CANCER VIRUSES
QUEST FOR HUMAN CANCER VIRUSES
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DOI：
10.1126/science.137.3533.835
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发表时间：
1962-01-01
期刊：
影响因子：
56.9
通讯作者：
YABE, Y
中科院分区：
文献类型：
--
作者：
TRENTIN, JJ;TAYLOR, G;YABE, Y
在这篇文章中提出了一种新的方法来揭示可能的人类肿瘤病毒这一重要而困难的任务。通过对已知的人类病毒致癌特性的系统测试，发现在出生后24小时内经肺内注射人12型腺病毒组织培养液的仓鼠，在1至3个月内注射部位的恶性肿瘤发生率很高。在HeLa细胞中，通过SELAS02过滤器的过滤或组织培养传代均不会丧失其致瘤活性。这样诱导的肿瘤在移植到的未受条件的年轻成年仓鼠中生长并死亡的比例很高。在注射了对照组织培养液或其他被测试病毒的培养液的仓鼠中，或者在对照种鼠中，没有发生这样的肿瘤。通过各种测试，明确排除了多瘤病毒和猿猴病毒40污染可能是诱发肿瘤的原因的可能性。通过肿瘤诱导能力与腺病毒含量的正相关，排除了其他未知污染病毒的可能参与。在测试的8种人血清中，只有中和腺病毒类型的细胞病变效应的4种血清也中和了诱发肿瘤的效应。在700份被测试的人血清中，26%的人含有滴度在14或更高的12型腺病毒的CPE中和抗体(23)。
A new approach to the important but difficult task of revealing possible human tumor viruses has been presented in this article. By systematic testing of already known human viruses for oncogenic properties, it was found that in hamsters injected intrapulmonarily with tissue culture fluid of human type 12 adenovirus within 24 hours after birth there was a very high incidence of malignant tumors at the site of injection in from 1 to 3 months. The tumor-inducing activity was not lost by filtration through Selas 02 filters or by tissue culture passages in He La cells. Tumors thus induced grew in, and killed, a high percentage of the unconditioned young adult hamsters into which they were transplanted. No such tumors occurred in hamsters injected with control tissue culture fluid or with culture fluids of the other viruses tested, or in control breeder hamsters. The possibility that contamination with polyoma virus and simian virus 40 might be responsible for the tumors induced was specifically excluded by a variety of tests. The possible involvement of still other, as yet unknown, contaminant viruses was excluded by a positive association of the tumor-inducing ability with the adenovirus content. Of eight human sera tested, only those four which neutralized the adenovirus-type cytopathic effect also neutralized the tumor-inducing effect. Of 700 human sera tested, 26 percent contained CPE-neutralizing antibodies for type 12 adenovirus at titers of 14 and higher (23).