Gene delivery using cell penetrating peptides-zeolitic imidazolate frameworks
Gene delivery using cell penetrating peptides-zeolitic imidazolate frameworks
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DOI：
10.1016/j.micromeso.2020.110173
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发表时间：
2020-06-15
影响因子：
5.2
通讯作者：
Langel, Ulo
中科院分区：
文献类型：
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作者：
Abdelhamid, Hani Nasser;Dowaidar, Moataz;Langel, Ulo
细胞穿透肽(CPPs)和金属有机骨架(MOF)有望成为下一代基因治疗药物的载体。报道了以寡核苷酸(ON)为载体的多孔分子筛咪唑骨架(ZIF-8)、氧化石墨烯(GO)、磁性纳米颗粒(MNPs)等纳米粒子在基因治疗中的组装。研究了5种不同类型的非病毒载体(ZIF-8、RhB@ZIF-8、BSA@ZIF-8、MNPs@ZIF-8和GO@ZIF-8)和3种基因治疗剂(质粒、剪接纠正寡核苷酸(SCO)和小干扰RNA(SiRNA))。对多聚体进行了表征，并将其应用于基因转染。该材料对荧光素酶的转导具有很低的毒性和很高的效率。ZIF-8可使CpP的质粒、SCO、siRNA的转染率提高2-8倍。还强调了细胞摄取的机制。数据揭示了通过A类清道夫(SCARA)进行的细胞内化。
Cell-penetrating peptides (CPPs), and metal-organic frameworks (MOFs) are promising as next-generation for the delivery of gene-based therapeutic agents. Oligonucleotide (ON)-mediated assembly of nanostructures composed of hierarchical porous zeolitic imidazolate framework (ZIF-8), and nanoparticles such as graphene oxide (GO), and magnetic nanoparticles (MNPs) for gene therapy are reported. Five different types of non-viral vectors (ZIF-8, RhB@ZIF-8, BSA@ZIF-8, MNPs@ZIF-8, and GO@ZIF-8), and three gene therapeutic agents (plasmid, splice correction oligonucleotides (SCO), and small interfering RNA (siRNA)) were investigated. The polyplexes were characterized and applied for gene transfection. The materials show very low toxicity with high efficiency for luciferase transfection. ZIF-8 enhances the transfection of plasmid, SCO, siRNA of CPPs by 2-8 folds. The mechanism of the cell uptakes was also highlighted. Data reveal cell internalization via scavenger class A (SCARA).