DEVELOPMENTALLY REGULATED EXPRESSION OF THE CELL CELL-ADHESION GLYCOPROTEIN CELL-CAM 120/80 IN PERIIMPLANTATION MOUSE EMBRYOS AND EXTRAEMBRYONIC MEMBRANES
DEVELOPMENTALLY REGULATED EXPRESSION OF THE CELL CELL-ADHESION GLYCOPROTEIN CELL-CAM 120/80 IN PERIIMPLANTATION MOUSE EMBRYOS AND EXTRAEMBRYONIC MEMBRANES
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DOI：
10.1016/0012-1606(86)90056-4
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发表时间：
1986-07-01
影响因子：
2.7
通讯作者：
DAMSKY, CH
中科院分区：
文献类型：
--
作者：
DAMJANOV, I;DAMJANOV, A;DAMSKY, CH
采用免疫组织化学方法检测小鼠胚周和胚外膜细胞-细胞粘附分子（cell-cell adhesion molecule, cell-CAM） 120/80的表达情况。细胞cam 120/80在囊胚内所有细胞的侧缘均可见，但在体外培养的滋养层巨细胞、部分单核滋养层细胞和卵黄囊壁细胞中未见。在体内的植入后胚胎中，除顶叶内胚层细胞和原代滋养层巨细胞外，早期卵筒的所有部分都能与细胞cam 120/80抗体发生反应。在卵筒后期，原始中胚层细胞和原始生殖细胞上未见细胞cam 120/80。生殖嵴和胎儿性腺的生殖细胞在整个胎儿发育阶段保持细胞cam 120/80阴性。胚胎外膜中，内脏卵黄囊、羊膜和胎盘迷路细胞cam 120/80阳性，而卵黄囊和海绵滋养层细胞cam 120/80阴性。这些数据表明，cam 120/80细胞存在于早期胚胎和胚胎外组织排列成上皮层的细胞中，但不存在于从这些上皮分化的游离细胞中。因此，细胞cam 120/80的表达似乎受到发育调控。
Peri-implantation mouse embryos and extraembryonic membranes were examined immunohistochemically for the expression of the cell-cell adhesion molecule (cell-CAM) 120/80. Cell-CAM 120/80 was seen along the lateral borders of all cells in the blastocyst but became undetectable on trophoblastic giant cells, some mononuclear trophoblastic cells and parietal yolk sac cells when blastocysts were cultured in vitro. In postimplantation embryos in vivo, all parts of the early egg-cyclinder reacted with the antibody to cell-CAM 120/80 except for the cells of the parietal endoderm and the primary trophoblastic giant cells. In the late stage egg-cylinder, no cell-CAM 120/80 was seen on the cells of the primitive mesoderm or on the primordial germ cells. The germ cells in genital ridges and fetal gonads remained cell-CAM 120/80-negative throughout the fetal stages of development. In the extraembryonic membranes, the visceral yolk sac, amnion, and the cells of the placental labyrinth were cell-CAM 120/80-positive, whereas, the parietal yolk sac cells and the spongiotrophoblast cells were negative. These data show that cell-CAM 120/80 is found on cells arranged into epithelial layers in the early embryo and extraembryonic tissues, but is not expressed in the dissociated cells differentiating from these epithelia. Thus, the expression of cell-CAM 120/80 appears to be developmentally regulated.