HUMAN IMMUNODEFICIENCY VIRUS-INFECTION OF CD4-BEARING CELLS OCCURS BY A PH-INDEPENDENT MECHANISM
HUMAN IMMUNODEFICIENCY VIRUS-INFECTION OF CD4-BEARING CELLS OCCURS BY A PH-INDEPENDENT MECHANISM
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DOI：
10.1002/j.1460-2075.1988.tb02839.x
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发表时间：
1988-02-01
期刊：
影响因子：
11.4
通讯作者：
WEISS, RA
中科院分区：
文献类型：
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作者：
MCCLURE, MO;MARSH, M;WEISS, RA
研究了弱碱（NH 4 Cl和金刚烷胺）和羧基离子载体（莫能菌素）对HIV-1感染CD 4（T4）阳性人细胞系的影响。这些提高酸性细胞内细胞器的pH的试剂在抑制低pH依赖性融合包膜病毒的浓度下不能抑制HIV-1进入和导致病毒抑制HIV-1进入的事件以及导致病毒蛋白合成的事件。VSV（HIV-1）假型的感染性不受弱碱的影响，弱碱浓度可使VSV自身包膜中的空斑减少95%。HIV-1依赖性细胞-细胞融合（合胞体形成）发生在维持在pH 7.4-7.6的培养基中，并且病毒体不会通过在酸性培养基中孵育而不可逆地失活。我们的研究结果表明，HIV-1的进入和膜融合不需要暴露在低pH值。然而，感染性HIV-1颗粒的产生，在用氯化铵处理的细胞中被抑制。
The effect of weak bases (NH4Cl and amantadine) and carboxylic ionophores (monensin) on the infection of CD4 (T4) positive human cell lines by HIV-1 is examined. These reagents, which raise the pH of acidic intracellular organelles, fail to inhibit HIV-1 entry and the events leading to viral to inhibit HIV-1 entry and the events leading to viral protein synthesis at concentrations inhibitory for low pH-dependent fusogenic enveloped viruses. The infectivity of VSV (HIV-1) psuedotypes is unaffected by weak bases at concentrations causing 95% plaque reduction of VSV in its own envelope. HIV-1 dependent cell-cell fusion (syncytium formation) occurs in medium maintained at pH 7.4-7.6, and virions are not irreversibly inactivated by incubation in acid medium. Our results show that HIV-1 entry and membrane fusion do not require exposure to low pH. The production of infectious HIV-1 particles, however, is inhibited in cells treated with NH4Cl.