The first milliseconds of the pore formed by a fusogenic viral envelope protein during membrane fusion.
The first milliseconds of the pore formed by a fusogenic viral envelope protein during membrane fusion.
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膜融合过程中融合病毒包膜蛋白形成的孔的第一毫秒。
DOI：
10.1073/pnas.88.9.3623
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发表时间：
1991
影响因子：
11.1
通讯作者：
Almers,W
中科院分区：
文献类型：
--
作者：
Spruce,AE;Iwata,A;Almers,W
在质膜上表达流感病毒血凝素的成纤维细胞在与红细胞融合时被膜片夹住。成纤维细胞膜电容的增加表明“融合孔”的打开，这是融合细胞之间的第一个水连接。我们在这里表明，电容增加之前是短暂的电流瞬态，当红细胞通过新生的融合孔释放其膜电位时产生。这个信号允许我们在孔隙存在的最初几毫秒内计算孔隙的电导。孔隙电导从0跳到大约150ps，然后在随后的几十毫秒内逐渐增长，直到增长停止。初始电导与大离子通道相似，表明孔洞初始宽度只有1-2纳米。因此，我们可能只观察到少数血凝素分子引起的事件。
Fibroblasts expressing the influenza virus hemagglutinin on their plasma membrane were patch clamped while they fused to erythrocytes. An increase in the fibroblast's membrane capacitance indicated the opening of the "fusion pore," the first aqueous connection between the fusing cells. We show here that the capacitance increase is preceded by a brief current transient, generated as the erythrocyte discharges its membrane potential through the nascent fusion pore. This signal allows one to calculate the pore conductance during the first milliseconds of its existence. The pore conductance jumps from 0 to approximately 150 pS and then grows more gradually over the subsequent tens of milliseconds until growth is arrested. The initial conductance is similar to that of a large ion channel and suggests that the pore is initially only 1-2 nm wide. Hence, we are probably observing events caused by only a small number of hemagglutinin molecules.