Transposition of the LINE-like retrotransposon TART to Drosophila chromosome termini.
Transposition of the LINE-like retrotransposon TART to Drosophila chromosome termini.
复制标题
将 LINE 样逆转录转座子 TART 转座至果蝇染色体末端。
DOI：
10.1073/pnas.91.26.12510
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发表时间：
1994
影响因子：
11.1
通讯作者：
Levis,RW
中科院分区：
文献类型：
--
作者：
Sheen,FM;Levis,RW
TART是一种来自果蝇的端粒相关DNA元件，在结构上与LINE （long interspersed element）样反转录转座子具有同源性，并转置到断裂的染色体末端。在染色体末端10个独立添加的DNA中，有7个通过原位杂交检测到TART DNA。我们发现证据表明，一个TART元素已经转置到染色体末端的每两个添加的详细检查。从最近转位的一个TART元件的DNA序列中，我们推断出TART编码的两种蛋白质与许多系的推定蛋白质具有显著的序列相似性。这些结果支持了一种假设，即作为维持果蝇端粒的基本过程的一部分，TART元件优先反转录到染色体末端。
TART, a telomere-associated DNA element from Drosophila, is shown in this paper to have structural homology to LINE (long interspersed element)-like retrotransposons and to transpose to broken chromosome ends. TART DNA was detected by in situ hybridization in 7 of 10 independent additions of DNA to a chromosome end. We found evidence that a TART element had transposed to the chromosome end in each of two additions that were examined in detail. From the DNA sequence of a TART element that recently transposed, we infer that TART encodes two proteins having significant sequence similarity to the putative proteins of many LINEs. These results support the hypothesis that TART elements preferentially retrotranspose to the termini of chromosomes as part of the essential process by which Drosophila telomeres are maintained.