Three-dimensional fluorescence imaging using the transport of intensity equation
Three-dimensional fluorescence imaging using the transport of intensity equation
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DOI：
10.1117/1.jbo.25.3.032004
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发表时间：
2019-11
影响因子：
3.5
通讯作者：
S. Rajput;Manoj Kumar;X. Quan;M. Morita;T. Furuyashiki;Y. Awatsuji;E. Tajahuerce;O. Matoba
中科院分区：
文献类型：
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作者：
S. Rajput;Manoj Kumar;X. Quan;M. Morita;T. Furuyashiki;Y. Awatsuji;E. Tajahuerce;O. Matoba
抽象。我们提出了一种非扫描三维（3-D）荧光成像技术，使用传输强度方程（TIE）和自由空间菲涅耳传播。在该成像技术中，通过基于TIE的相位恢复算法来恢复与具有点光源状形状的散焦荧光图像相对应的相位分布。从所获得的散焦荧光图像的相位分布及其对应的振幅分布，在复波函数的菲涅耳传播之后，可以在期望的平面处重建不同距离处的各种图像。通过所提出的成像方法，可以在多个平面中执行3-D荧光成像。荧光强度图像是在电可调透镜的帮助下捕获的;因此，成像技术没有运动伪影。我们目前的实验结果对应的微珠和生物样品，以证明建议的3-D荧光成像技术。
Abstract. We propose a nonscanning three-dimensional (3-D) fluorescence imaging technique using the transport of intensity equation (TIE) and free-space Fresnel propagation. In this imaging technique, a phase distribution corresponding to defocused fluorescence images with a point-light-source-like shape is retrieved by a TIE-based phase retrieval algorithm. From the obtained phase distribution, and its corresponding amplitude distribution, of the defocused fluorescence image, various images at different distances can be reconstructed at the desired plane after Fresnel propagation of the complex wave function. Through the proposed imaging approach, the 3-D fluorescence imaging can be performed in multiple planes. The fluorescence intensity images are captured with the help of an electrically tunable lens; hence, the imaging technique is free from motion artifacts. We present experimental results corresponding to microbeads and a biological sample to demonstrate the proposed 3-D fluorescence imaging technique.