A New Strategy of Using Keratinocytes for Skin Graft: Animal Experimental Study of Keratinocyte Suspension Combined with the Dermal Substitutes
A New Strategy of Using Keratinocytes for Skin Graft: Animal Experimental Study of Keratinocyte Suspension Combined with the Dermal Substitutes
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DOI：
10.1159/000210672
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发表时间：
2009-04
影响因子：
1.6
通讯作者：
Shi-chu Xiao;S. H. Zhu;H. Y. Li;J. Yang;Z. F. Xia
中科院分区：
文献类型：
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作者：
Shi-chu Xiao;S. H. Zhu;H. Y. Li;J. Yang;Z. F. Xia
目的：为皮肤角质形成细胞移植提供一种新的、简便的方法。方法：制备角质形成细胞悬液及脱细胞真皮基质（ADM）、胶原膜等真皮替代物。建立Balb/c-nu小鼠全层皮肤缺损创面，分为5组：对照组，单纯移植角质形成细胞悬液;两步ADM+胶原膜组，ADM或胶原膜移植后2周滴入角质形成细胞悬液。观察上皮细胞百分率。结果如下：移植后2周，一步法ADM组未见再上皮化迹象，一步法胶原膜组、二步法ADM组和二步法胶原膜组的再上皮化率分别为54.1%、41.8%和76.8%。表明二步法胶原膜组的再上皮化率高于其他两组（P < 0.01）。结论：角质形成细胞悬液联合真皮替代物移植是一种更为灵活的利用角质形成细胞的方法。与ADM相比，微孔胶原膜与角质形成细胞悬液联合应用于皮肤移植是一种更好的选择。
Objective: To provide a new and simple strategy for keratinocyte transplantation. Methods: Keratinocyte suspension and dermal substitutes including acellular dermal matrix (ADM) and collagen membrane were prepared. Full-thickness skin defect wounds of Balb/c-nu mice were created and the mice were assigned into 5 groups: the control group, where the wound was grafted with keratinocyte suspension only; the one-step ADM and collagen membrane groups, where the wound was grafted with ADM or collagen membrane plus dripping of keratinocyte suspension; the two-step ADM and collagen membrane groups, where keratinocyte suspension was dripped 2 weeks after ADM or collagen membrane grafting. The percentage of epithelium was observed. Results: Two weeks after grafting, no evidence of re-epithelialization was seen in the one-step ADM group, and the re-epithelialization rate in the one-step collagen membrane group, two-step ADM group and two-step collagen membrane group was 54.1, 41.8 and 76.8%, respectively, indicating that the re-epithelialization rate in the two-step collagen membrane group was higher than that in the other two groups (p < 0.01). Conclusions: Keratinocyte suspension combined with dermal substitutes grafting offers a more flexible way of making use of keratinocytes. Compared with ADM, microporous collagen membrane is a more preferable option for combined use with keratinocyte suspension for skin grafting.