Endothelial cell cycle kinetics. Changes in culture and correlation with endothelial properties.
Endothelial cell cycle kinetics. Changes in culture and correlation with endothelial properties.
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内皮细胞周期动力学。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
A. Yen
中科院分区：
文献类型：
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作者：
J. Goldsmith;J. J. McCormick;A. Yen
培养的内皮细胞已成为评价内皮特性的模型系统。培养条件可能影响这些内皮特性或生长特征的表达。因此，我们评估了细胞周期动力学，前列环素释放，血管紧张素转换酶活性在原代和传代培养的细胞从牛肺和全身血管。前列环素释放是高度可变的，在文化中通过。然而，用离子载体A23187刺激后的释放随着连续传代而下降。血管紧张素转换酶活性也随着反复传代培养而下降。超过10%的原代融合牛内皮细胞在循环，而人脐静脉内皮细胞为2 - 8%。随着培养物中的传代，25%的牛内皮细胞在汇合时循环。偶尔传代培养的牛内皮细胞株停滞在G2，但在传代培养后会重新进入G1。暴露于胰蛋白酶似乎不会导致这种现象。这些研究已经证明了在体外繁殖过程中内皮细胞特性和生长特征的变异性。在研究传代培养内皮细胞的特性或反应时，必须考虑这种变异性的存在。
Endothelial cells in culture have emerged as a model system for evaluation of endothelial properties. Culture conditions may affect expression of these endothelial properties or growth characteristics. Therefore, we evaluated cell cycle kinetics, prostacyclin release, and angiotensin-converting enzyme activity in primary and subcultured cells from bovine pulmonary and systemic vessels. Prostacyclin release was highly variable during passage in culture. However, release following stimulation with ionophore A23187 declined with serial passage. Angiotensin-converting enzyme activity also declined with repeated subculturing. Greater than 10% of primary confluent bovine endothelial cells were cycling compared to 2 to 8% of human umbilical vein endothelial cells. With passage in culture, 25% of bovine endothelial cells were cycling at confluence. Occasional strains of subcultured bovine endothelium arrested in G2 but would reenter G1 upon subculturing. Exposure to trypsin did not appear to contribute to this phenomenon. These studies have demonstrated variability in endothelial cell properties and growth characteristics during in vitro propagation. The existence of this variability must be considered when studying properties or responses of subcultured endothelium.