Isolation and characterization of novel marine-derived actinomycete taxa rich in bioactive metabolites
Isolation and characterization of novel marine-derived actinomycete taxa rich in bioactive metabolites
复制标题
DOI：
10.1128/aem.70.12.7520-7529.2004
复制
发表时间：
2004-12-01
影响因子：
4.4
通讯作者：
Sherman, DH
中科院分区：
文献类型：
--
作者：
Magarvey, NA;Keller, JM;Sherman, DH
一个独特的选择性富集程序导致分离和鉴定两个新属的海洋来源放线菌。用该方法培养的微生物约90%来自潜在的新属，结果表明其选择性高。在这项研究中，从巴布亚新几内亚海岸的俾斯麦海和所罗门海的潮下海洋沉积物中分离出102种放线菌。结合生理参数、化学分类特征、16S rRNA基因序列的区分以及基于16S rRNA基因的系统发育分析，为小单孢科两个新属（以PNG1枝和UMM518为代表）的存在提供了强有力的证据。新型海洋放线菌发酵产物的生物活性测试显示，一些放线菌具有抗多药阳性病原体、恶性细胞和痘苗病毒复制的活性。
A unique selective enrichment procedure has resulted in the isolation and identification of two new genera of marine-derived actinobacteria. Approximately 90% of the microorganisms cultured by using the presented method were from the prospective new genera, a result indicative of its high selectivity. In this study, 102 actinomycetes were isolated from subtidal marine sediments collected from the Bismarck Sea and the Solomon Sea off the coast of Papua New Guinea. A combination of physiological parameters, chemotaxonomic characteristics, distinguishing 16S rRNA gene sequences, and phylogenetic analysis based on 16S rRNA genes provided strong evidence for the two new genera (represented by strains of the PNG1 clade and strain UMM518) within the family Micromonosporaceae. Biological activity testing of fermentation products from the new marine-derived actinomycetes revealed that several had activities against multidrug-resistant grant-positive pathogens, malignant cells, and vaccinia virus replication.